Generic placeholder image

Current Enzyme Inhibition


ISSN (Print): 1573-4080
ISSN (Online): 1875-6662

Research Article

Screening of Actinomycetes For α-amylase Inhibitors Production

Author(s): Shivabai Chandwad* and Sudhakar Gutte

Volume 15 , Issue 1 , 2019

Page: [41 - 45] Pages: 5

DOI: 10.2174/1573408015666190110130544

Price: $65


Background: Diabetes mellitus is the most common and fastest growing disease in the world. One of the therapies to treat diabetes is the inhibition of α-amylase activity by inhibitors from microbial and plant source. Actinomycetes are potential sources of enzyme inhibitors, drugs, amino acids, vitamins etc.

Objective: Our work mainly highlights the isolation of actinomycetes from soil samples of different habitats and screening of α -amylase inhibitors.

Methods: Actinomycetes were isolated from soil samples of different habitats by different methods; these include a variety of pre-treatment of soil samples in combination with an appropriate supplement medium with selective antibacterial agents. Isolated actinomycetes grown in fermentation condition and metabolites were extracted with Isopropyl alcohol and concentrated to obtain solid material. The extract of each isolate was tested for α -amylase inhibition using starch Iodine plate method and DNS- spectroscopic method.

Results: Total 110 actinomycetes strains were isolated from various sources. Among 110 extracts of actinomycetes, eight extracts have shown positive results for α-amylase inhibition in starch Iodine plate assay method. Extracts selected from primary results were used for the confirmation of inhibitory activity using DNS- spectroscopic method. Out of eight extracts, six extracts showed Porcine pancreatic α -amylase inhibitory activity ranging from 40-86%. The actinomycetes strains that produce α -amylase inhibitory activity are A-24, A-29, B-5, B-18, C-15 and D-24.

Conclusion: These results show that actinomycetes are a potential source for α -amylase inhibitors, which may lead to valuable novel drugs for diabetic treatment.

Keywords: Actinomycetes, amylase, diabetics, inhibitors, α-amylase, bioactive compounds.

Graphical Abstract
Tamotsu, F.; Yasuhiro, I.; Takako, I.; Tomomitsu, S.; Noriko, S.; Ryuji, Y. Isolation of actinomycetes from live plants and evaluation of antiphytopathogenic activity of their metabolites. Actinomycetol., 2002, 16, 9-13.
Umezawa, H.; Okami, T.; Hashimoto, T.; Suhara, Y.; Hamada, M.; Takeuchi, T. A new antibiotic, kasugamycin. J. Antibiot. Ser. A., 1965, 18, 101-103.
Chiaki Imada. 2005 Enzyme inhibitors and other bioactive compounds from marine actinomycetes Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology. 4-5-7.
Matsukawa, M.; Nakagawa, Y.; Limura, Y.; Hayakawa, M. A new enrichment method for the selective isolation of streptomycetes from the root surfaces of herbaceous plants. Actinomycetologica, 2007, 21, 66-69.
Hong, K.; Gao, A.; Xie, Q.; Gao, H.; Zhuang, L.; Lin, H.; Yu, H.; Li, J.; Yao, X.; Goodfellow, M.; Ruan, J. Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. Mar. Drugs, 2009, 7, 24-44.
Hayakawa, M.; Tamura, T.; Iino, H.; Nonomura, H. Pollenbaiting and drying method for highly selective isolation of Actinoplanes spp. from soil. J. Ferment. Bioeng., 1991, 72, 433-438.
Tamura, T.; Hayakawa, M.; Hatano, K. A new genus of the order Actinomycetales, Spirilliplanes gen. nov., with description of Spirilliplanesyamanashiensis sp. nov. Int. J. Syst. Bacteriol., 1997, 47, 97-102.
Hayakawa, M. Studies on the isolation and distribution of rare actinomycetes in soil. Actinomycetologica, 2008, 22, 12-19.
Hirsch, C.; Christensen, D. Novel method for selective isolation of actinomycetes. Appl. Environ. Microbiol., 1983, 46, 925-929.
Bredholt, H.; Fjaervik, E.; Jhonsen, G.; Zotechev, S.B. Actinomycetes from sediments in the TrondheinFjrod, Norway: Diversity and biological activity. Mar. Drugs, 2008, 6, 12-24.
Ravi, R.K.; Vasantba, J.J. Isolation of Actinomycetes: A complete approach. Int. J. Curr. Microbiol. App. Sci., 2016, 5(5), 606-618.
Tsao, P.; Leben, C.; Keitt, G. An enrichment method for isolating actinomycetes that produce diffusible antifungal antibiotic. Pytopathol., 1960, 50, 88-89.
El-Nakeeb, M.; Lechevalier, H. Selective isolation of aerobic actinomycetes. Appl. Microbiol., 1963, 11, 75-77.
Lawrence, C. A method of isolating actinomycetes from scabby potato tissue and soil with minimal contamination. Can. J. Bot., 1956, 34, 44-47.
Hayakawa, M.; Iino, H.; Takeuchi, S.; Yamazaki, T. Application of a method incorporating treatment with Chloramine-T for the selective isolation of Streptosporangiaceae from soil. J. Ferment. Bioeng., 1997, 84, 599-602.
Hayakawa, M.; Sadakata, T.; Kajiura, T.; Nonomura, H. New methods for the highly selective isolation of Micromonospora and Microbispora from soil. J. Ferment. Bioeng., 1991, 72, 320-326.
Hayakawa, M. Nonomura. H. A new method for the intensive isolation of actinomycetes from soil. Actinomycetol., 1989, 3, 95-104.
Hayakawa, M.; Yoshida, Y.; Iimura, Y. Selective isolation of bioactive soil actinomycetes belonging to the Streptomyces violaceusniger phenotypic cluster. J. Appl. Microbiol., 2004, 96, 973-981.
Miiller, G.L. Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Anal. Chem., 1959, 31, 426-428.
Sharma, M.; Dangi, P.; Choudhary, M. Actinomycetes: Source, identification, and their applications. Int. J. Curr. Microbiol. Appl. Sci., 2014, 3(2), 801-832.
Hayakawa, M.; Tamura, T.; Iino, H.; Nonomura, H. New methods for the highly selective isolation of Streptosporangium and Dactylosporangium from soil. J. Ferment. Bioeng., 1991, 72, 327-333.
Yamamura, H.; Hayakawa, M.; Iimura, Y. Application of sucrose-gradient centrifugation for selective isolation of Nocardia spp. from soil. J. Appl. Microbiol., 2003, 95, 677-685.
Yamamura, H.; Hayakawa, M.; Nakagawa, Y.; Iimura, Y. Species diversity of Nocardiae isolated from Lake and Moat sediment samples. Actinomycetol., 2003, 17, 44-46.
Dulaney, E.; Larsen, A.; Stapley, E. A note on the isolation of microorganisms from natural sources. Mycologia, 1955, 47, 420-422.
Corke, C.; Chase, I. Comparative studies of actinomycete populations in acid podzolic and neutral mull forest soils. Soil Sei. SOC. Amer. Proc., 1964, 28, 68-70.
Porter, J.; Wilhelm, J.; Tresner, H. Method for the preferential isolation of actinomycetes from soils. Appl. Microbiol., 1960, 8, 174-178.
Corbaz, R.; Gregoby, P.; Lacey, M. Thermophilic and mesophilic actinomycetes in mouldy hay. J. Gen. Microbiol., 1963, 32, 449-456.
Cross, T. Actinomycetes: A continuing source of new metabolites. Dev. Indust. Microbiol., 1982, 23, 1-18.
Goodfellow, M.; Donnel, A. Search and discovery of industrially significant actinomycetes. In microbial products: New approaches, society for general microbiology symposium no. 44; Baumberg, S.; Hunter, I.S., and ; Rhodes, P.M., Eds.; 343-383. Cambridge: Cambridge University Press 1989
Linagappa, Y.; Lockwood, J. Chitin media for selective isolation and culture of actinomycetes. Phytopathol, 1962, 52, 317-323.
Porter, J.; Wilhelm, J.; Tresner, H. Method for the preferential isolation of actinomycetes from soils. Appl. Microbiol., 1960, 8, 174-178.
Xiao, Z.; Storms, R.; Tsang, A. A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Anal. Biochem., 2006, 351, 146-148.

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy