摘要
背景:多梳抑制复合物2(PRC 2)催化H3 Lys 27的组蛋白甲基化,在许多系统的发育和疾病过程中起着至关重要的作用。它的催化亚基EZH 2是长非编码RNA(IncRNAs)的关键核靶点,它是一种新兴的表观遗传调节剂,参与多种细胞过程。INcRNA具有较高的组织特异性,但对EZH 2相互作用的IncRNAs的组织特征了解甚少。目的:采用RNA免疫沉淀和RNA测序相结合的方法,对脑、肺、心、肝、肾、肠、脾、睾丸、肌肉和血液等组织中EZH 2结合的INcRNAs进行全局筛选。我们鉴定了1328个EZH_2结合的INcRNAs,其中470个在至少两个组织中共享,858个在单个组织中被检测到。在许多incRNAs中发现了一个具有特殊二级结构的RNA基序,尽管不是所有EZH 2结合的incRNAs。EZH 2结合的INcRNA可分为4类:基因间INcRNA、反义INcRNA、内含子相关INcRNA和启动子相关INcRNA。启动子相关的INcRNA Hnf1aos1与肝脏中的EZH 2特异结合,与其配对编码基因HNF1A具有相同的特征,进一步证实了本研究的有效性。除了已知的EZH 2结合的INcRNAs,如Kcnq1ot1、Gas5、Meg3、Hotair和MALAT 1之外,大多数的INcRNAs被报道与EZH 2有关。结论:我们的发现提供了EZH 2-相互作用于不同组织的INcRNAs的剖面图,并提示了INcRNAs在细胞分化和成熟过程中的关键作用。表观遗传学,长非编码RNA,组织特异性,PRC 2,EZH 2,组蛋白甲基化
关键词: 表观遗传学,长非编码RNA,组织特异性,PRC 2,EZH 2,组蛋白甲基化
Current Gene Therapy
Title:EZH2 RIP-seq Identifies Tissue-specific Long Non-coding RNAs
Volume: 18 Issue: 5
关键词: 表观遗传学,长非编码RNA,组织特异性,PRC 2,EZH 2,组蛋白甲基化
摘要: Background: Polycomb Repressive Complex 2 (PRC2) catalyzes histone methylation at H3 Lys27, and plays crucial roles during development and diseases in numerous systems. Its catalytic subunit EZH2 represents a key nuclear target for long non-coding RNAs (lncRNAs) that emerging to be a novel class of epigenetic regulator and participate in diverse cellular processes. LncRNAs are characterized by high tissue-specificity; however, little is known about the tissue profile of the EZH2- interacting lncRNAs.
Objective: Here we performed a global screening for EZH2-binding lncRNAs in tissues including brain, lung, heart, liver, kidney, intestine, spleen, testis, muscle and blood by combining RNA immuno- precipitation and RNA sequencing. We identified 1328 EZH2-binding lncRNAs, among which 470 were shared in at least two tissues while 858 were only detected in single tissue. An RNA motif with specific secondary structure was identified in a number of lncRNAs, albeit not in all EZH2-binding lncRNAs. The EZH2-binding lncRNAs fell into four categories including intergenic lncRNA, antisense lncRNA, intron-related lncRNA and promoter-related lncRNA, suggesting diverse regulations of both cis and trans-mechanisms. A promoter-related lncRNA Hnf1aos1 bound to EZH2 specifically in the liver, a feature same as its paired coding gene Hnf1a, further confirming the validity of our study. In addition to the well known EZH2-binding lncRNAs like Kcnq1ot1, Gas5, Meg3, Hotair and Malat1, majority of the lncRNAs were firstly reported to be associated with EZH2.
Conclusion: Our findings provide a profiling view of the EZH2-interacting lncRNAs across different tissues, and suggest critical roles of lncRNAs during cell differentiation and maturation.
Export Options
About this article
Cite this article as:
EZH2 RIP-seq Identifies Tissue-specific Long Non-coding RNAs, Current Gene Therapy 2018; 18 (5) . https://dx.doi.org/10.2174/1566523218666181008125010
DOI https://dx.doi.org/10.2174/1566523218666181008125010 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
The link between Hepatic Vitamin A Metabolism and Nonalcoholic Fatty Liver Disease
Current Drug Targets In Vitro and In Vivo Antitumor Efficacy of Docetaxel and Sorafenib Combination in Human Pancreatic Cancer Cells
Current Cancer Drug Targets Progress and Prospects of Stem Cells in Treatment of Drug Resistant Tuberculosis
Current Respiratory Medicine Reviews Gallium-68 in Medical Imaging
Current Radiopharmaceuticals Attenuation of Hydroxyl Radical Formation by Extracted Constituent of Moringa oleifera Lam
Current Chemical Biology Herbal Remedies for the Treatment of Periodontal Disease - A Patent Review
Recent Patents on Drug Delivery & Formulation Management of Glioblastoma Multiforme by Phytochemicals: Applications of Nanoparticle-Based Targeted Drug Delivery System
Current Drug Targets Molecular Mechanisms of Biological Activity of Oleanolic Acid - A Source of Inspiration for A New Drugs Design
Mini-Reviews in Organic Chemistry Proteomics-Based Characterization of the Effects of MMP14 on the Protein Content of Exosomes from Corneal Fibroblasts
Protein & Peptide Letters Difference Gel Electrophoresis: Application in Quantitative Proteomics Research
Current Proteomics The Immunomodulatory Potential of Selected Bioactive Plant-Based Compounds in Breast Cancer: A Review
Anti-Cancer Agents in Medicinal Chemistry 1,2,3-Triazine Scaffold as a Potent Biologically Active Moiety: A Mini Review
Mini-Reviews in Medicinal Chemistry Role of Antimicrobial Peptides Expressed by Host Cells Upon Infection by Helicobacter pylori
Protein & Peptide Letters Molecular Target-Guided Tumor Therapy with Natural Products Derived from Traditional Chinese Medicine
Current Medicinal Chemistry Probiotics for the Prevention and Treatment of Allergies, with an Emphasis on Mode of Delivery and Mechanism of Action
Current Pharmaceutical Design An Update on the Other Telomerase Inhibitors: Non-G-Quadruplex Interactive Agent, Non-Antisense, Non-Reverse Transcriptase Telomerase Inhibitors
Medicinal Chemistry Reviews - Online (Discontinued) Nitric Oxide and Inflammation
Inflammation & Allergy - Drug Targets (Discontinued) 1,3,5-Triazine Analogs: A Potent Anticancer Scaffold
Current Signal Transduction Therapy Repurposing Drugs for Cancer Prevention
Current Topics in Medicinal Chemistry miR-149 as a Potential Molecular Target for Cancer
Current Medicinal Chemistry