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Current Cancer Therapy Reviews


ISSN (Print): 1573-3947
ISSN (Online): 1875-6301

Research Article

Chitosan Nanoparticles as a Carrier for Indigofera intricata Plant Extract: Preparation, Characterization and Anticancer Activity

Author(s): Aliasgar Shahiwala*, Naglaa G. Shehab, Maryam Khider and Rawoof Khan

Volume 15, Issue 2, 2019

Page: [162 - 169] Pages: 8

DOI: 10.2174/1573394714666181008112804

Price: $65


Background: Cancer is one of the major causes of the death and affects people of all ages throughout the world. The drugs that are currently available to treat cancer have many side effects. Hence, there is considerable scientific interest in the continuing discovery of new anticancer agents from natural sources. The aim of this study was to prepare and characterize nanoparticles combining Indigofera intricata crude alcoholic extract and chitosan and to evaluate the anticancer cell proliferative activity for both extract and nanoparticles.

Methods: Dried alcoholic extract was prepared and characterized for its phenolic and flavonoid contents. Chitosan extract nanoparticles was prepared by ionic gelation method and characterized by thin layer chromatography (TLC), Fourier-transform infrared spectroscopy (FTIR), particle size and zeta-potential analysis. The anticancer cell proliferative activities of both plant extract and nanoparticles at different concentrations were evaluated using breast cancer cell line (MCF 7).

Results: The alcoholic extract showed high contents from both phenolic and flavonoid constituents (15 % and 22 % respectively). The interaction of polyphenolic compounds of the extract with chitosan was confirmed by the TLC and FTIR results. The particle size and zeta-potential of nanoparticles found to be 400.6nm ± 101.8 nm and +42.1 mV ± 9.27 mV respectively. The plant extract showed the lowest cell viability of 45.21% ± 4.8% at the highest dose (250 mg) tested in this investigation. Almost 500-fold reduction (from 250 mg to 0.5 mg) in the extract concentration required to achieve same anticancer cell proliferative activity when formulated as nanoparticles. Also 2.5 mg extract containing nanoparticles showed similar anticancer cell proliferative activity as 5 mg 5-FU.

Conclusion: Our results revealed that traditional medicinal plants could be an excellent source of natural anticancer agents and the chitosan-extract nanoparticles is a promising formulation strategy to enhance their clinical effectiveness.

Keywords: Indigofera intricata, polyphenolic compounds, flavonoids, MCF 7, chitosan nanoparticles, anticancer cell proliferative activity.

Graphical Abstract
FastStats-Leading causes of death. Accessed on: February 4, 2018. Available from:
Cragg GM, Newman DJ. Plants as a source of anti-cancer agents Accessed on: February 4, 2018. Available from:.
Paulino J, Groppo M, Teixeira S. Floral developmental morphology of three Indigofera species (Leguminosae) and its systematic significance within Papilionoideae. Plant Syst Evol 2011; 292(3): 165-76.
Han R. Highlight on the studies of anticancer drugs derived from plants in China. Stem Cells 1996; 12: 53-63.
Rahman TU, Zeb MA, Liaqat W, Sajid M, Hussain S, Choudhary MI. Phytochemistry and pharmacology of genus Indigofera: A review. Rec Nat Prod 2018; 12(1): 1-13.
Azzi J, Tang L, Moore R, et al. Polylactide-cyclosporin a nanoparticles for targeted immunosuppression. FASEB J 2010; 24(10): 3927-38.
Barbucci R, Lamponi S, Magnani A, Peluso G, Petillo O. Metal complexes with linear and crosslinked polysaccharides as mediators of angiogenesis. Polym Adv Technol 2001; 12(3-4): 271-8.
British Pharmacopoeia Ash Value, Acid Insoluble, Water Soluble Extractive and Alcohol Extractive Vol 2 Appendix Xii Majesty Stationary Office London. 1276-9.1980;
Oktay M, İlhami G, Küfrevioğlu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum Vulgare) seed extracts. Food Sci Technol 2003; 36(2): 263-71.
Dewanto V, Xianzhong W, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 2002; 50(10): 3010-4.
Grenha A. Chitosan nanoparticles: A survey of preparation methods. J Drug Target 2012; 20(4): 291-300.
Al-Qubaisi MS, Rasedee A, Flaifel MH, et al. Induction of apoptosis in cancer cells by NiZn Ferrite nanoparticles through mitochondrial cytochrome C release. Int J Nanomedicine 2013; 8: 4115-30.
Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981-2002. J Nat Prod 2003; 66(7): 1022-37.
Munin A, Edwards-Lévy F. Encapsulation of natural polyphenolic compounds: A review. Pharmaceutics 2011; 3(4): 793-829.
Bansal SS, Goel M, Aqil F, Vadhanam MV, Gupta RC. Advanced drug delivery systems of curcumin for cancer chemoprevention. Cancer Prev Res 2011; 4(8): 1158-71.
Coimbra M, Isacchi B, van Bloois L, et al. Improving solubility and chemical stability of natural compounds for medicinal use by incorporation into liposomes. Int J Pharm 2011; 416(2): 433-42.
Shoji Y, Nakashima H. Nutraceutics and delivery systems. J Drug Target 2004; 12(6): 385-91.
Zhou Y, Zheng J, Li Y, et al. Natural polyphenols for prevention and treatment of cancer. Nutrients 2016; 8(8)E515
Hu B, Pan C, Sun Y, et al. Optimization of fabrication parameters to produce chitosan−tripolyphosphate nanoparticles for delivery of tea catechins. J Agric Food Chem 2008; 56(16): 7451-8.
Syaefudin . Juniarti A, Rosiyana L, Setyani A, Khodijah S. Nanoparticles of selaginella doederleinii leaf extract inhibit human lung cancer cells A549. Avialble from: (accessed on: February 4, 2018).
Liang J, Li F, Fang Y, et al. Synthesis, characterization and cytotoxicity studies of chitosan-coated tea polyphenols nanoparticles. Colloids Surf B Biointerfaces 2011; 82(2): 297-301.
Stoica R, Şomoghi R, Ion RM. Preparation of chitosan- tripolyphosphate nanoparticles for the encapusulation of polyphenols extracted from rose hips. Dig J Nanomater Biostruct 2018; 8(3): 955-63.
Erbacher P, Zou S, Steffan AM, Remy JS. Chitosan-based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability. Pharm Res 1998; 15: 1332-9.
Tiyaboonchai W. Chitosan nanoparticles: A promising system for drug delivery. Naresuan Univ J 2003; 11(3): 51-66.
Sun G, Zhang S, Xie Y, Zhang Z, Zhao W. Gallic acid as a selective anticancer agent that induces apoptosis in SMMC-7721 human hepatocellular carcinoma cells. Oncol Lett 2016; 11(1): 150-8.
Pasut G, Canal F, Via LD, Arpicco S, Veronese FM, Schiavon O. Antitumoral activity of PEG–gemcitabine prodrugs targeted by folic acid. J Control Release 2008; 127(3): 239-48.
Müller RH, Jacobs C. Buparvaquone mucoadhesive nanosuspension: Preparation, optimisation and long-term stability. Int J Pharm 2002; 237(1-2): 151-61.

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