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Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Letter Article

Identification and Characterization of Genes in the Curcuminoid Pathway of Curcuma zedoaria Roscoe

Author(s): Truong Thi Phuong Lan, Nguyen Duc Huy, Nguyen Ngoc Luong, Nguyen Van Nghi, Trinh Huu Tan, Le Viet Quan and Nguyen Hoang Loc*

Volume 19, Issue 10, 2018

Page: [839 - 846] Pages: 8

DOI: 10.2174/1389201019666181008112244

Price: $65

Abstract

Background: Curcuminoid genes have an important role in the biosynthesis of curcumin, a valuable bioactive compound, in Curcuma species. However, there have not been any reports of these genes in Curcuma zedoaria.

Objective: The present work reports on the isolation of genes encoding enzymes in curcuminoid metabolic pathway and their expression in C. zedoaria.

Method: The primers were designed from untranslation regions of DCS, CURS1, CURS2 and CURS3 genes which are involved in curcuminoid biosynthesis in C. longa to isolate the corresponding fulllength genes in C. zedoaria. RT-PCR amplification and HPLC analysis are used to estimate the expression of genes and biosynthesis of curcumin in both rhizome and callus.

Results: The results showed that all four genes from C. zedoaria (named CzDCS, CzCURS1, CzCURS2 and CzCURS3) and C. longa have a high identity (approximately 99%) and lengths of genes from C. zedoaria are 1382, 1240, 1288 and 1265 nu, respectively. CzCURS1, 2 and 3 genes have one intron while CzDCS has two introns. RT-PCR amplification indicated that curcuminoid genes expressed mRNA in rhizome and callus of C. zedoaria. Curcumin, a major component of curcuminoids, was also found in callus by HPLC analysis.

Conclusion: The sequence information of DCS and CURS1-3 genes in C. zedoaria will be very valuable for a subsequent study on the effects of elicitors on the transcription of genes involved in curcuminoid biosynthesis pathway.

Keywords: Curcuma zedoaria, callus, curcumin, curcumin synthase genes, curcuminoid metabolic pathway, diketide-CoA synthase gene.

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