Login Register Cart 0
Generic placeholder image

Letters in Drug Design & Discovery

Editor-in-Chief

ISSN (Print): 1570-1808
ISSN (Online): 1875-628X

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Phenolic Composition and Antifungal Effect of Costus cf. arabicus L Against Yeast of the Candida Genus

Author(s): Antonia Thassya Lucas Dos Santos, Antonio Júdson Targino Machado, Maria Audilene De Freitas, Irwin Rose Alencar De Menezes, Henrique Douglas Melo Coutinho, Francisco Assis Bezerra Da Cunha, Djair Dos Santos De Lima E Souza*, Aline Augusti Boligon, Adeliana Silva De Oliveira, Andressa De Alencar Silva and Maria Flaviana Bezerra Morais-Braga*

Volume 16, Issue 5, 2019

Page: [502 - 511] Pages: 10

DOI: 10.2174/1570180815666180827122526

Price: $65

Abstract

Background: The emergence of fungal resistance to commercial drugs has been observed, and because of that, research with natural products have been performed with the aim of obtaining bioactive compounds.

Objective: Evaluate the chemical composition and antifungal activity of the ethanolic extract of Costus cf. arabicus L leaves (EECAL) over strains of the genus Candida, as well as its inhibitory potential over yeast virulence.

Methods: The composition of EECAL was analyzed through High Performance Liquid Chromatography (HPLC). The Minimum Inhibitory Concentration (MIC) was determined by broth microdilution using spectrophotometer readings and the Minimal Fungicidal Concentration (MFC) was investigated. The reading data of the MIC was used to trace a cellular growth curve and calculate the Inhibitory Concentration for 50% of the cells (IC50) of the extract and fluconazole. The effect over the yeast morphology was verified using wet-chamber microculture and visualized through optical microscopy (40x).

Results: HPLC detected the presence of flavonoids and phenolic acids. The extract presented fungistatic effect (MIC of 8.192 µg/mL). The IC50 of the extract and fluconazole varied between 4,008.7 to 5,116.8 µg/mL and 44.0 to 83.1 µg/mL, respectively. The extract inhibited the formation of hyphae at MICx2 against CA LM 77. For the CA INCQS 40006, the inhibition was verified at MIC/2. In the CT LM 23 and CT INCQS 40042 strains the presence of hyphae was considered absent at the MIC.

Conclusion: The extract presented antifungal action on cell growth at elevated concentrations and an inhibitory effect of dimorphism in the tested Candida species.

Keywords: Candida albicans, Candida tropicalis, costaceae, virulence inhibition, fluconazole, dimorphism.

« Previous Next »
Graphical Abstract

[1]
Menezes, E.A.; Cunha, M.D.; Ferreira, É.B.; Capelo, L.G.; Braz, B.H.L.; Cunha, F.A. Perfil de suscetibilidade de Candida tropicalis a antifúngicossistêmicos. Rev. Pathol. Trop., 2013, 42, 49-55.
[http://dx.doi.org/10.5216/rpt.v42i1.23598]
[2]
De Rossi, T.; Lozovoy, M.A.B.; Da Silva, R.V.; Fernandes, E.V.; Geraldino, T.H.; Costa, I.C. Sar Idakis, H.O.; Watanabe, M.A.E.; Felipe, I. Interações entre Candida albicans e hospedeiro, Semin. Ciências Biológic. da Saúde 2011, 32, 15-28.
[3]
Correia, A.F.; Silveira, D.; Fonseca-Bazzo, Y.M.; Magalhães, P.O.; Fagg, C.W.; Da Silva, E.C.; Gomes, S.M.; Gandolfi, L.; Pratesi, R.; Medeiros-Nóbrega, Y.K. Activity of crude extracts from Brazilian cerrado plants against clinically relevant Candida species. BMC Complement. Altern. Med., 2016, 16, 203.
[4]
Pfaller, M.A. Antifungal drug resistance: mechanisms, epidemiology, and consequences for treatment. Am. J. Med., 2012, 125, S3-S13.
[5]
López, C.A.A. Considerações gerais sobre plantas medicinais. Ambient. Gestão E Desenvolv. 2006, 1, 19-27.
[6]
Heinzmann, B.M.; De Barros, F.M.C. Potencial das plantasnativasbrasileiraspara o desenvolvimento de fitomedicamentostendocomoexemploLippia alba (Mill.) NE Brown (Verbenaceae). Saúde (Santa Maria), 2007, 33, 43-48.
[7]
Specht, C.D.; Kress, W.J.; Stevenson, D.W.; DeSalle, R. A molecular phylogeny of Costaceae (Zingiberales). Mol. Phylogenetics. Evol., 2001, 21, 333-345.
[8]
Lans, C.; Harper, T.; Georges, K.; Bridgewater, E. Bridgewater, Medicinal and ethnoveterinary remedies of hunters in Trinidad. BMC Complement. Altern. Med., 2001, 1, 1.
[9]
Mosihuzzaman, M.; Nahar, N.; Ali, L.; Rokeya, B.; Khan, A.K.; Nur-E-Alam, M.; Nandi, R.P. Hypoglycemic effects of three plants from eastern himalayan belt. Diabetes Res., 1994, 26, 127-138.
[10]
Teixeira, S.A.; Melo, J.I.M. Plantasmedicinaisutilizadas no município de Jupi, Pernambuco, Brasil. Iheringia Série Botânica., 2006, 61, 5-11.
[11]
Castro, J.A.; Brasileiro, B.P.; Lyra, D.H.; De Almeida Pereira, D.; Chaves, J.L. Ethnobotanical study of traditional uses of medicinal plants: The flora of caatinga in the community of Cravolndia-BA, Brazil. J. Med. Plants Res., 2011, 5, 1905-1917.
[12]
Pereira, R.P.; Boligon, A.A.; Appel, A.S.; Fachinetto, R.; Ceron, C.S.; Tanus-Santos, J.E.; Athayde, M.L.; Rocha, J.B.T. Chemical composition, antioxidant and anticholinesterase activity of Melissa officinalis. Ind. Crops Prod., 2014, 53, 34-45.
[13]
Boligon, A.A.; Kubiça, T.F.; Mario, D.N.; De Brum, T.F.; Piana, M.; Weiblen, R.; Lovato, L.; Alves, S.H.; Santos, R.C.V.; Dos Santos Alves, C.F. Antimicrobial and antiviral activity-guided fractionation from ScutiabuxifoliaReissek extracts. Acta Physiol. Plant., 2013, 35, 2229-2239.
[14]
De Souza, A.B.; Souza, L.M.S.; Carvalho, J.C.T.; Maistro, E.L. No clastogenic activity of Caesalpiniaferrea Mart.(Leguminosae) extract on bone marrow cells of Wistar rats. Genet. Mol. Biol., 2006, 29, 380-383.
[15]
Javadpour, M.M.; Juban, M.M.; Lo, W.C.J.; Bishop, S.M. Bishop, J.B. Alberty, S.M. Cowell, C.L. Becker, M.L. McLaughlin, De novo antimicrobial peptides with low mammalian cell toxicity. J. Med. Chem., 1996, 39, 3107-3113.
[16]
Ernst, E.J.; Klepser, M.E.; Ernst, M.E.; Messer, S.A.; Pfaller, M.A. In vitropharmacodynamic properties of MK-0991 determined by time-kill methods. Diagn. Microbiol. Infect. Dis., 1999, 33, 75-80.
[17]
Sidrim, J.J.C.; Rocha, M.F.G. Micologia médica à luz de autores contemporâneos. Guanabara Koogan: Rio de Janeiro 2008.
[18]
Mendes, J.M. Investigação da AtividadeAntifúngica do ÓleoEssencial de Eugenia caryophyllataThunb. sobrecepas de Candida tropicalis, Dissertação; Universidade Federal da Paraíba: João Pessoa, 2011.
[19]
Machado, H.; Nagem, T.J.; Peters, V.M.; Fonseca, C.S. De Oliveira, T.T. Flavonóides e seupotencialterapêutico, Bol. Do Cent. Biol. Da Reprodução., 2008, 27, 33-39.
[20]
Dar, K.B.; Bhat, A.H.; Amin, S.; Anees, S.; Masood, A.; Zargar, M.I.; Ganie, S.A. Efficacy of Aqueous and Methanolic Extracts of Rheum spiciformis against Pathogenic Bacterial and Fungal Strains. J. Clin. Diagn. Res., 2016, 10, BC18-BC22.
[21]
López-Posadas, R.; Ballester, I.; Abadía-Molina, A.C.; Suárez, M.D.; Zarzuelo, A.; Martínez-Augustin, O.; De Medina, F.S. Effect of flavonoids on rat splenocytes, a structure-activity relationship study. Biochem. Pharmacol., 2008, 76, 495-506.
[22]
Cherrak, S.A. Mokhtari-Soulimane, N.; Berroukeche, F.; Bensenane, B.; Cherbonnel, A.; Merzouk, H.; Elhabiri, M. In Vitro antioxidant versus metal ion chelating properties of flavonoids: A structureactivity investigation.PLoS One; , 2016, 11, p. 10, e0165575..
[23]
Oliveira, M.S.; Furlong, E.B. Screening of antifungal and antimycotoxigenic activity of plant phenolic extracts. World Mycotoxin J., 2008, 1(2), 139-146.
[24]
Bitencourt, A.P.R. Estudo fitoquímico, toxicológico e microbiológico das folhas de Costus spicatus. Jacq. Biota Amaz. (Biote Amaz. Biota Amaz. Amaz. Biota)., 2014, 4, 75-79.
[25]
Cunha, F.A.B.; Matias, E.F.F.; Brito, S.V.; Ferreira, F.S.; Braga, J.M.A.; Costa, J.G.M.; Coutinho, H.D.M. In vitro antibacterial, phototoxic, and synergistic activity of ethanol extracts from Costus cf. arabicusL. J. Med. Food, 2011, 14, 964-968.
[26]
Cunha, F.A.B.; Matias, E.F.F.; Brito, S.V.; Ferreira, F.S.; Braga, J.M.A.; Costa, J.G.M.; Coutinho, H.D.M. Phytochemical screening, antibacterial activity and in vitro interactions between Costus cf. arabicusL. with UV-A and aminoglycosides. Nat. Prod. Res., 2012, 26, 380-386.
[27]
Souza, C.E.S. FigueiredoLeite, N.; Vieira de Brito, D.I.; Batista Alencar, L.B.; Lima Santos de Lavor, A.K.; Ferreira Matias, E.F.; Sabino Albuquerque, R.; De Alencar Ferreira, J.V.; Bezerra da Cunha, F.A.; RelisonTintino, S. Evaluación de la actividadantimicrobiana y modulación del extracto de hexano de Costus cf. Arabicus L. Rev. Cuba. Plantas Med., 2014, 19, 151-159.
[28]
Tintino, S.R.; Da Cunha, F.A.B.; Dos Santos, K.K.A. De MeloGuedes, G.M.; Souza, C.E.S.; Matias, E.F.F.; Morais-Braga, M.F.B.; Andrade, J.C.; Da Costa, J.G.M.; De Freitas, M.A. Atividademoduladora de extratosetanólico e hexânico de raiz de Costus cf. arabicussobredrogasantimicrobianas. Rev. Bras. Biociencias, 2013, 11.
[29]
Tintino, S.R.; Guedes, G.M.M.; Da Cunha, F.A.B.; Dos Santos, K.K.A.; Matias, E.F.F.; Morais-Braga, M.F.B.; Andrade, J.C.; Souza, E.S.; De Freitas, M.A.; Alencar, L.B.B. AvaliaçãoIn vitro da atividadeantimicrobiana e moduladora dos extratosetanólico e hexânico de bulbo de Costusarabicus= In vitro evaluation of antimicrobial activity and modulating the ethanol and hexane extracts of Costusarabicus bulb. Biosci. J., 2013, 29, 723-738.
[30]
Mayer, F.L.; Wilson, D.; Hube, B. Candida albicans pathogenicity mechanisms. Virulence, 2013, 4, 119-128.
[31]
Ribeiro, E.L. Leveduras de Candidaisoladas da boca de crianças com e semSíndrome de Down : aspectosfeno-genotípicos, relaçãointrafamilial e perfil de imunoglobulinasLeveduras de Candidaisoladas da bocade crianças com e semSíndrome de Down : aspectosfeno-genot, Tese; Universidade Federal de Goiás, 2008.
[32]
Jacobsen, I.D.; Wilson, D.; Wächtler, B.; Brunke, S.; Naglik, J.R.; Hube, B. Candida albicans dimorphism as a therapeutic target. Expert Rev. Anti Infect. Ther., 2012, 10, 85-93.
[33]
Alves, L.A.; Freires, I. de A.; Pereira, T.M.; De Souza, A.; Lima, E. de O.; De Castro, R.D. Effect of Schinusterebinthifolius on Candida albicans growth kinetics, cell wall formation and micromorphology. Acta Odontol. Scand., 2013, 7, 965-971.
[34]
Van Burik, J-A.H.; Magee, P.T. Aspects of fungal pathogenesis in humans. Annu. Rev. Microbiol., 2001, 55, 743-772.
[35]
Salari, S.; Bakhshi, T.; Sharififar, F.; Naseri, A.; Almani, P.G.N. Evaluation of antifungal activity of standardized extract of Salvia rhytideaBenth. (Lamiaceae) against various Candida isolates.J. Mycol. Médicale J. Med. Mycol., 2016.
[36]
Bound, D.J.; Murthy, P.S.; Srinivas, P. 2, 3-Dideoxyglucosides of selected terpene phenols and alcohols as potent antifungal compounds. Food Chem., 2016, 210, 371-380.
[37]
Sharma, A.; Gangwar, M.; Kumar, D.; Nath, G.; Sinha, A.S.; Tripathi, Y. Phytochemical characterization, antimicrobial activity and reducing potential of seed oil, latex, machine oil andpresscake of Jatrophacurcas. Avicenna J. Phytomedicine., 2016, 6, 366-375.
[38]
Longhini, R.; Raksa, S.M.; Oliveira, A.C.P.; Svidzinski, T.I.E.; Franco, S.L. Obtenção de extratos de própolis sob diferentescondições e avaliação de suaatividadeantifúngica. Rev. Bras. Farm., 2007, 17, 388-395.
[39]
Silva, M.V.B. Efeito da adição de compostos fenólicos sobre o crescimento de levedura etanologênica (Saccharomyces sp.) em meio sintético e em caldo de cana-de-açúcar. 2013.
[40]
Candiracci, M.; Citterio, B.; Piatti, E. Antifungal activity of the honey flavonoid extract against Candida albicans. Food Chem., 2012, 131, 493-499.

Rights & Permissions Print Cite
Article Metrics
37
4
1
1
© 2024 Bentham Science Publishers | Privacy Policy