Abstract
With the near availability of a cloned Caenorhabditis elegans ORFeome, the development of a high-throughput worm interactome mapping project has now become feasible. After reviewing the increasing interest for C. elegans as a model organism, from ”classical“ biology to the post-genomic era, this article presents the early steps accomplished for the generation of a C. elegans protein interaction map using the twohybrid system. Different approaches have been examined in order to determine the appropriate strategy to screen protein-protein interactions on a large scale. Our version of the two-hybrid system has been used to generate prototype maps for 4 different C. elegans biological modules (vulval development, proteasome, DNA repair / cell cycle and dauer). Those pilot projects helped us developing experimental procedures to avoid most false positive protein interactions while covering a satisfactory proportion of geniune interactions. In silico analysis and experimental validation of the potential protein interactions have already demonstrated biological relevance for some of the networks of interactions obtained.
Keywords: Caenorhabditis elegans orfeome
Current Genomics
Title: The Caenorhabditis elegans Interactome Mapping Project
Volume: 3 Issue: 2
Author(s): Jean-Francois Rual, Philippe Lamesch, Jean Vandenhaute and Marc Vidal
Affiliation:
Keywords: Caenorhabditis elegans orfeome
Abstract: With the near availability of a cloned Caenorhabditis elegans ORFeome, the development of a high-throughput worm interactome mapping project has now become feasible. After reviewing the increasing interest for C. elegans as a model organism, from ”classical“ biology to the post-genomic era, this article presents the early steps accomplished for the generation of a C. elegans protein interaction map using the twohybrid system. Different approaches have been examined in order to determine the appropriate strategy to screen protein-protein interactions on a large scale. Our version of the two-hybrid system has been used to generate prototype maps for 4 different C. elegans biological modules (vulval development, proteasome, DNA repair / cell cycle and dauer). Those pilot projects helped us developing experimental procedures to avoid most false positive protein interactions while covering a satisfactory proportion of geniune interactions. In silico analysis and experimental validation of the potential protein interactions have already demonstrated biological relevance for some of the networks of interactions obtained.
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Cite this article as:
Rual Jean-Francois, Lamesch Philippe, Vandenhaute Jean and Vidal Marc, The Caenorhabditis elegans Interactome Mapping Project, Current Genomics 2002; 3 (2) . https://dx.doi.org/10.2174/1389202024605206
DOI https://dx.doi.org/10.2174/1389202024605206 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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