Generic placeholder image

Current Nanomedicine


ISSN (Print): 2468-1873
ISSN (Online): 2468-1881

Research Article

Development and Evaluation of Lycopene Loaded Chitosan Nanoparticles

Author(s): A. Dhiman* and D. Bhalla

Volume 9 , Issue 1 , 2019

Page: [61 - 75] Pages: 15

DOI: 10.2174/2468187308666180815145855

Price: $65


Background: Nanotechnology has gained a great deal of public interest due to the needs and applications of nanomaterials in many areas of human endeavours such as industry, agriculture, business, medicine and public health amongst many others. Polymeric nanoparticles from biodegradable and biocompatible polymers are good candidates for drug carrier to deliver the drugs because they are expected to be adsorbed in an intact form in the gastrointestinal tract after oral administration.

Objective: The objective of the study was to investigate the influence of some precarious variables like, concentration of chitosan, concentration of sodium tripolyphosphate (STPP) and stirring time on physicochemical characteristics of lycopene loaded chitosan nanoparticles.

Method: Eight batches of lycopene loaded chitosan nanoparticles were prepared using various concentrations of chitosan (100-200 mg), STPP (50-100 mg) by varying stirring speed in the range of 10-20 minutes using ionic gelation method. The optimized nanoparticulate formulation was characterized for various parameters like morphology study, particle size and size distribution studies, differential scanning calorimetry, entrapment efficiency and in-vitro drug release studies.

Results: Lycopene loaded chitosan nanoparticles containing 150 mg of chitosan, 75 mg of STPP, 20 mg of drug lycopene and with 15 min of stirring time showed entrapment efficiency of 89.4%. The percent release of lycopene loaded chitosan nanoparticles at the end of 6 h was found to be 83.5%, however, percent release of pure lycopene at the end of 6 h was observed as 79.6%.

Conclusion: Lycopene loaded chitosan nanoparticles may show a great promise for the development of drug delivery system by enhancing the cellular accumulation of lycopene with chitosan.

Keywords: Chitosan, ionic gelation method, lycopene, nanoparticles, sodium tripolyphosphate (STPP), nanotechnology.

Graphical Abstract
Strambeanu N, Demetrovici L, Dragos D, Lungu M. Lungu, M, Neculae, A, Bunoiu, M, Biris, C, Eds Nanoparticles’ promises and risks nanoparticles: definition, classification and general physical properties. Switzerland: Springer International Publishing 2015; pp. 3-8.
Bhilana S, Dhiman A, Singh G, Satija S. Gas chromatography-mass spectroscopy analysis of bioactive compounds in the whole plant parts of ethanolic extract of Asclepias curassavica L. Int J Green Pharm 2018; 2: 107-14.
Tiwari G, Tiwari R, Sriwastawa B, et al. Drug delivery systems: An updated review. Int J Pharm Investig 2012; 2(1): 2-11.
Dhiman B. Divtrannum, Dhiman A, Saini S. An appraisal on various methods of nano particulate formulations. Pharm Nanotech 2017; 5(4): 255-62.
Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS. Polymeric scaffolds in tissue engineering application: A review. Int J Polymer Sci 2011; 2011: 1-19.
Wang JJ, Zeng ZW, Xiao RZ, et al. Recent advances of chitosan nanoparticles as drug carriers. Int J Nanomedicine 2011; 6: 765-74.
Lu Z, Chen W, Hamman JH. Chitosan - polycarbophil complexes in swellable matrix systems for controlled drug release. Curr Drug Deliv 2007; 4(4): 257-63.
Elumalai AM, Karthika BB, Usha V. Lycopene - role in cancer prevention. Int J Pharma Bio Sci 2013; 4(3): 371-8.
Beer O, Zofia P. Lycopene (synthetic) chemical and technical assessment (CTA) Office of food additive safety. Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration : College Park, Maryland, USA 2006; pp. 1-6.
Eh A, Teoh SG. Novel modified ultrasonication technique for the extraction of lycopene from tomatoes. Ultrasonics Sonochem 2012; 19: 151-9.
Aghel N, Ramezani Z, Amirfakhrian S. Isolation and quantification of lycopene from tomato cultivated in dezfoul, Iran. J Natural Pharm Prod 2011; 6(1): 9-15.
Hosniyeh H, Fatemeh A, Rassoul D, Sayed O. Chitosan-Pluronic nanoparticles as oral delivery of anticancer gemcitabine, preparation and in-vitro study. Int J Nanomed 2012; 7: 1851-63.
Aminabhavi T, Agnihotri S, Mallikarjuna N. Recent advances on chitosan-based micro and nanoparticles in drug delivery. J Control Rel 2004; 100(1): 5-28.
Dhiman A, Singh D. Development, characterization & in-vitro skin permeation of rutin ethosomes as a novel vesicular carrier. Int J Biomed Res 2013; 4(10): 559-66.
Thi V, Anh H, Saehoon K, et al. Antioxidant activity and bioaccessibility of size-different nanoemulsion for lycopene-enriched tomato extract. Food Chem 2015; 178: 115-21.
Shi XY, Fan XG. Advances in nanoparticle system for delivering drugs across the biological barriers. J China Pharm Univ 2002; 33: 169-72.
Wang JJ, Zeng ZW, Xiao RZ, Xie T, Zhou GL, Zhan XR, et al. Recent advances of chitosan nanoparticles drug carriers. Int J Nanomed 2011; 6: 765-74.
Dhiman A, Sharma K, Sharma A, Sindhu P. A review on the status of quality control and standardization of herbal drugs in India. Drug Dev Ther 2016; 7(2): 107-12.
Dhiman A, Nanda A, Ahmad S. A quest for staunch effects of flavonoids: Utopian protection against hepatic ailments. Arab J Chem 2016; 9: S1813-23.
Sodde V, Dashora N, Prabhu KS, Lobo R. Evaluation of anticancer activity of Macrosolen parasiticus (L.) danser on Ehrlich’s ascites carcinoma treated mice. Int J Cancer Res 2011; 7(2): 135-43.
Saini S, Dhiman A, Nanda S. Pharmacognostical and phytochemical studies of Piper betle Linn. leaf. Int J Pharm Pharm Sci 2016; 8(5): 222-6.

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy