Now a days, nanotechnology has emerged as the promising area which controls the matters at the scale of nano ranges i.e. from 1–100 nm especially deals with nanoparticles. These nanoparticles might be formulated by applying a variety of approaches like physical, chemical as well as biological approach. The physical and chemical approach is time-consuming and uses toxic chemicals which require a lot of energy for synthesis. Nevertheless, the biological method uses a variety of biological organisms like bacteria, fungi and plant as nanofactories and is easily available, very easy to handle and least toxic. These particles have a wide range of applicability in a range of sectors of life especially in the field of medical sciences. Current review gives some idea of silver nanoparticles obtained through biological resources which are environmental friendly and their potential application in a variety of areas of life sector.