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Current Bioactive Compounds

Editor-in-Chief

ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

General Research Article

Maximized Extraction of Flavonoid Luteolin from V.negundo L. Leaves: Optimization Using Box-Behnken Design

Author(s): Lubna Abidin, Mohammad Mujeeb* and Showkat R. Mir

Volume 15, Issue 3, 2019

Page: [343 - 350] Pages: 8

DOI: 10.2174/1573407214666180731120014

Price: $65

Abstract

Background: Luteolin is a flavonoid unveiling various therapeutic activities, found in Vitex negundo L. Thus, there is a need to present process parameters at which maximum amount of luteolin can be extracted from V. negundo L. leaves in “one-run”.

Objective: Response surface methodology (RSM) was employed for optimizing the process parameters for the extraction of luteolin from V. negundo L. leaves. The study also compared the efficacy of various traditional and modern extraction methods for luteolin extraction.

Methods: Extraction conditions (solvent to drug ratio, extraction temperature and extraction time) were optimized by RSM, Box-Behnken Design (BBD). Quantification of luteolin in various extracts was done through High Performance Liquid Chromatography (HPLC).

Results: Hot solvent extraction by reflux technique stood out to be the best technique and methanol was found to be the most effective solvent for luteolin extraction.Through the use of BBD, the optimal conditions for luteolin extraction were established as: solvent to drug ratio- 17.7 mL/g, extraction temperature- 55.5°C and extraction time-2.04 hours. Under such conditions 7.32 %w/w of luteolin was yielded which was close to predicted value of 7.29 %w/w.

Conclusion: Reflux technique stood out to be the best among all the studied modes of extraction and methanol proved to be the most effective solvent. Moreover, all the three variables significantly affected the luteolin extraction. Our study shows the applicability of a statistical technique, RSM in phytocompound extraction field. This makes the optimization technique cheap and less laborious than the traditional optimization method.

Keywords: Response surface methodology, high performance liquid chromatography, polyphenol, anti-oxidant , Hot solvent extraction, Nirgundi.

Graphical Abstract
[1]
Zhu, T.; Row, K.H. Box-behnken design for optimizing extraction of luteolin from celery leaves. J. Liq. Chrom. Rel. Techno., 2011, 34(12), 1036-1049.
[2]
Kumar, S.; Pandey, A.K. Chemistry and biological activities of flavonoids: an overview. Sci. World J., 2013, 2013, 162750.
[3]
Kim, J.S.; Kwon, C.S.; Son, K.H. Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid. Biosci. Biotechnol. Biochem., 2000, 64(11), 2458-2461.
[4]
Abidin, L.; Mujeeb, M.; Imam, S.S.; Aqil, M.; Khurana, D. Enhanced transdermal delivery of luteolin via nonionic surfactant-based vesicle: quality evaluation and anti-arthritic assessment. Drug Deliv., 2015, 21, 1-6.
[5]
Maruyama, S.A.; Palombini, S.V.; Claus, T.; Carbonera, F.; Montanher, P.F.; de Souza, N.E.; Visentainer, J.V.; Gomes, S.T.M.; Matsushita, M. Application of box-behnken design to the study of fatty acids and antioxidant activity from enriched white bread. J. Braz. Chem. Soc., 2013, 24(9), 1520-1529.
[6]
Cotelle, N.; Bernier, J.L.; Catteau, J.P.; Pommery, J.; Wallet, J.C.; Gaydou, E.M. Antioxidant properties of hydroxy-flavones. Free Radic. Biol. Med., 1996, 20(1), 35-43.
[7]
Fotsis, T.; Pepper, M.S.; Aktas, E.; Breit, S.; Rasku, S.; Adlercreutz, H.; Wähälä, K.; Montesano, R.; Schweigerer, L. Flavonoids, dietary-derived inhibitors of cell proliferation and in vitro angiogenesis. Cancer Res., 1997, 57(14), 2916-2921.
[8]
Hiremath, S.P.; Badami, S.; Hunasagatta, S.K.; Patil, S.B. Antifertility and hormonal properties of flavones of Striga orobanchioides. Eur. J. Pharmacol., 2000, 391(1-2), 193-197.
[9]
Kim, J.S.; Kwon, C.S.; Son, K.H. Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid. Biosci. Biotechnol. Biochem., 2000, 64(11), 2458-2461.
[10]
Simoes, C.M.; Schenkel, E.P.; Bauer, L.; Langeloh, A. Pharmacological investigation on Achyrocline satureioides (Lam.) D.C. Compositae. Ethanopharmacol., 1988, 22(3), 28-293.
[11]
Wang, J.; Zhang, J.; Zhao, B.; Wang, X.; Wu, Y.; Yao, J. A comparison study on microwave-assisted extraction of Potentilla anserine L. polysaccharides with conventional methods: Molecular weight and anti-oxidant activities evaluation. Carbohydr. Polym., 2010, 80, 84-93.
[12]
Alam, M.S.; Damanhouri, Z.A.; Ahmad, A.; Abidin, L.; Amir, M.; Aqil, M.; Khan, S.A.; Mujeeb, M. Development of response surface methodology for optimization of extraction parameters and quantitative estimation of embelin from Embelia ribes Burm by high performance liquid chromatography. Pharmacogn. Mag., 2015, 11(1)(Suppl. 1), S166-S172.
[13]
Bulduk, I.; Gezer, B.; Cengiz, M. Optimization of ultrasound-assisted extraction of morphine from capsules of Papaver somniferum by response surface methodology. Int. J. Anal. Chem., 2015, 2015, 796349.
[14]
Ghasemzadeh, A.; Jaafar, H.Z.; Karimi, E.; Rahmat, A. Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology. BMC Complement. Altern. Med., 2014, 14, 318.
[15]
Siddiqui, N.; Aeri, V. Optimization of betulinic acid extraction from Tecomella undulate bark using a box-behnken design and its densitometric validation. Molecules, 2016, 21(4), 393.
[16]
Cristea, D.; Bareau, I.; Gerard, V. Identification and quantitative HPLC analysis of the main flavonoids present in weld (Reseda luteola L.). Dyes Pigm., 2003, 57, 267-272.
[17]
Nath, A.; Chattopadhyay, P.K. Optimization of oven toasting for improving crispness and other quality attributes of ready to eat potato soy snack using response surface methodology. J. Food Eng., 2007, 80, 1282-1292.
[18]
Shailajan, S.; Yeragi, M. Optimization of microwave assisted extraction of luteolin from leaves of Vitex negundo Linn and its comparison with conventional extraction methods. Int. J. Pharma. Res. Dev., 2011, 3(5), 128-134.

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