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Current Analytical Chemistry


ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Research Article

Graphene Modified Molecular Imprinting Electrochemical Sensor for Determining the Content of Dopamine

Author(s): Rong Liu, Jie Li, Tongsheng Zhong and Liping Long*

Volume 15 , Issue 6 , 2019

Page: [628 - 634] Pages: 7

DOI: 10.2174/1573411014666180730112304

Price: $65


Background: The unnatural levels of dopamine (DA) result in serious neurological disorders such as Parkinson’s disease. Electrochemical methods which have the obvious advantages of simple operation and low-cost instrumentation were widely used for determination of DA. In order to improve the measurement performance of the electrochemical sensor, molecular imprinting technique and graphene have always been employed to increase the selectivity and sensitivity.

Methods: An electrochemical sensor which has specific selectivity to (DA) was proposed based on the combination of a molecular imprinting polymer (MIP) with a graphene (GR) modified gold electrode. The performance and effect of MIP film were investigated by differential pulse voltammetry (DPV) and cyclic voltammetry (CV) in the solution of 5.0 ×10-3 mol/L K3[Fe(CN)6] and K4[Fe(CN)6] with 0.2 mol/L KCl at room temperature.

Results: This fabricated sensor has well repeatability and stability, and was used to determine the dopamine of urine. Under the optimized experiment conditions, the current response of the imprinted sensor was linear to the concentration of dopamine in the range of 1.0×10-7 ~ 1.0×10-5 mol/L, the linear equation was I (µA) = 7.9824+2.7210lgc (mol/L) with the detection limit of 3.3×10-8 mol/L.

Conclusion: In this work, a highly efficient sensor for determination of DA was prepared with good sensitivity by GR and great selectivity of high special recognization ability by molecular imprinting membrane. This proposed sensor was used to determine the dopamine in human urine successfully.

Keywords: Dopamine, gold electrode, graphene, molecularly imprinted membrane, parkinson’s disease, differential pulse voltammetry (DPV).

Graphical Abstract
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