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The Natural Products Journal

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ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

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Research Article

Phytochemical Profiles, Antioxidant and Antibacterial Activities of 11 Phellinus Mushrooms Collected in Thailand

Author(s): Punjaporn Sunthudlakhar, Pongtip Sithisarn*, Bhusita Wannissorn, Siripen Jarikasem and Piyanuch Rojsanga

Volume 9, Issue 2, 2019

Page: [144 - 156] Pages: 13

DOI: 10.2174/2210315508666180621160917

Price: $65

Abstract

Background: Phellinus mushrooms are locally available in Thailand. They have been traditionally used for medicinal purposes including tonics, for cancer treatment and for immune system stimulation.

Objective: To evaluate the in vitro antioxidant, antibacterial activities and the phytochemical information of the extracts from 11 selected Phellinus mushrooms collected in Thailand.

Methods: Eleven Phellinus mushrooms were collected from the Northeastern part of Thailand. Their ethanol and water extracts were tested for in vitro antioxidant activities using a DPPH scavenging assay, FRAP assay and TBARS method while the antibacterial effects were determined by agar disk diffusion and broth microdilution methods. The phytochemical profiles of the Phellinus mushroom extracts were evaluated using TLC and HPLC techniques. The total phenolic and total flavonoid contents of the extracts were evaluated by Folin-Ciocalteu and aluminium chloride methods, respectively. While total carbohydrate and total triterpene contents were evaluated by phenolsulfuric acid and vanillin perchloric acid methods, respectively.

Results: Most ethanol extracts from Phellinus mushrooms exhibited moderate antioxidant effects especially extracts from P. everhartii, P. hippophaëicola and P. pini var. microporus while P. igniarius var. cinereus ethanol extract exhibited inhibitory effects to Helicobacter pylori both normal and resistant strains, this extract also showed the strongest inhibitory effect against Staphylococcus aureus. Most Phellinus mushroom extracts exhibited similar TLC and HPLC fingerprints with the chromatographic bands corresponding to phenolics, flavonoids and terpenoids. Protocatechuic acid was identified in most Phellinus mushroom extracts.

Conclusion: Phellinus mushrooms especially, P. everhartii could be developed as sources of antioxidants while P. igniarius var. cinereus indicated the potential to inhibit H. pylori. Protocatechuic acid could be used as a marker for quality control of Phellinus mushroom raw materials and extracts.

Keywords: Phellinus, antioxidant, antibacterial, total phenolic, total flavonoid, HPLC, protocatechuic acid.

Graphical Abstract

[1]
Ruggiero, M.A.; Gordon, D.P.; Orrell, T.M.; Bailly, N.; Bourgoin, T.; Brusca, R.C.; Cavalier-Smith, T.; Guiry, M.D.; Kirk, P.M. A higher level classification of all living organisms. PLoS One, 2015, 10(4), 1-60.
[2]
Wasser, S.P. Reishi. In:Encyclopedia of Dietary Supplements; Coates, P.M.; Betz, J.M.; Blackman, M.R.; Cragg, G.M.; Levine, M.; Moss, J.; White, J.D., Eds.; Informa Healthcare: London, 2010, pp. 680-690.
[3]
Feeney, M.J.; Dwyer, J.; Hasler-Lewis, C.M.; Milner, J.A.; Noakes, M.; Rowe, S.; Wach, M.; Beelman, R.B.; Caldwell, J.; Cantorna, M.T.; Castlebury, L.A.; Chang, S.; Cheskin, L.J.; Clemens, R.; Drescher, G.; Fulgoni, III, V.L.; Haytowitz, D.B.; Hubbard, V.S.; Law, D.; Miller, A.M.; Minor, B.; Percival, S.S.; Riscuta, G.; Schneeman, B.; Thornsbury, S.; Toner, C.D.; Woteki, C.E.; Wu, D. Mushrooms and health summit proceedings. J. Nutr., 2014, 1128-1136.
[4]
Rathore, H.; Prasad, S.; Sharma, S. Mushroom nutraceuticals for improved nutrition and better human health: A review. Pharm. Nutr., 2017, 5, 35-46.
[5]
Paterson, R.R. Ganoderma - A therapeutic fungal biofactory. Phytochemistry, 2006, 67, 1985-2001.
[6]
Chen, H.; Tian, T.; Miao, H.; Zhao, Y. Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus: A review. Fitoterapia, 2016, 113, 6-26.
[7]
Zhu, T.; Kim, S.; Chen, C. A medicinal mushroom: Phellinus linteus. Curr. Med. Chem., 2008, 15, 1330-1335.
[8]
Lee, I.; Yun, B. Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance. J. Antibiot., 2011, 64, 349-359.
[9]
Soković, M.; Glamočlija, J.; Ćirić, A.; Petrović, J.; Stojkovic, D. Mushrooms as Sources of Therapeutic Foods In: Therapeutic foods -Handbook of Food Bioengineering Volume 8; Holban, A.M.; Grumezescu, A.M., Ed., Academic Press: San Diego, 2017, pp. 141-178.
[10]
Zhang, L.; Reddy, N.; Koyyalamudi, S. Isolation, characterization, and biological activities of polysaccharides from medicinal plants and mushrooms. In:Studies in Natural Products Chemistry. volume 42; Rahman, A., Ed.; Elsevier Publication: Amsterdam, 2014, pp. 117-151.
[11]
Chen, Y.; Chang, H.; Deng, J.; Chen, J.; Huang, S.; Lin, I.; Kuo, W.; Chao, W.; Huang, G. Hispolon from Phellinus linteus induces G0/G1 cell cycle arrest and apoptosis in NB4 human leukaemia cells. Am. J. Chin. Med., 2013, 41(6), 1439-1457.
[12]
Chen, W.; Zhao, Z.; Li, L.; Wu, B.; Chen, S.; Zhou, H.; Wang, Y.; Li, Y. Hispolon induces apoptosis in human gastric cancer cells through a ROS-mediated mitochondrial pathway. Free Radic. Biol. Med., 2008, 45, 60-72.
[13]
Chang, H.; Sheu, M.; Yang, C.; Lu, T.; Chang, Y.; Peng, W.; Huang, S.; Huang, G. Analgesic effects and the mechanisms of anti-inflammation of hispolon in mice. Evid. Based Complement. Alternat. Med., 2011, 1-8.
[14]
De Silva, D.D.; Rapior, S.; Hyde, K.D.; Bahkali, A.H. Medicinal mushrooms in prevention and control of diabetes mellitus. Fungal Divers., 2012, 56, 1-29.
[15]
Liu, H.; Tsai, T.; Chang, T.; Chou, C.; Lin, L. Lanostane-triterpenoids from the fungus Phellinus gilvus. Phytochemistry, 2009, 70, 558-563.
[16]
Klinhom, U. Medicinal mushrooms. In:Moh-Chao-Ban. volume 327; Vasri, P., Ed.; Moh-Chao-Ban Publishing House: Bangkok, 2007, pp. 17-24.
[17]
Chandrasrikul, A. Checklist of mushrooms (Basidiomycetes) in Thailand; Office of Natural Resources and Environmental Policy and Planning: Bangkok, 2011.
[18]
Larsen, M.J.; Cobb-Poulle, L.A. Phellinus (Hymenochaetaceae)- A survey of the world taxa; Fungiflora: Oslo, 1990.
[19]
Ellis, M.B.; Ellis, J.P. Fungi without Gills (Hymenomycetes and Gasteromycetes)- An Identification Handbook; Chapman and Hall: London, 1991.
[20]
Dai, Y.; Zhou, L.; Cui, B.; Chen, Y.; Decock, C. Current advances in Phellinus sensu lato: Medicinal species, functions, metabolites and mechanisms. Appl. Microbiol. Biotechnol., 2010, 87, 1587-1593.
[21]
Sithisarn, P.; Rojsanga, P.; Sithisarn, P.; Kongkiatpaiboon, S. Antioxidant activity and antibacterial effects on clinical isolated Streptococcus suis and Staphylococcus intermedius of extracts from several parts of Cladogynos orientalis and their phytochemical screenings. Evid. Based Complement. Alternat. Med., 2015, 1-5.
[22]
Ferreira, I.; Baptista, P.; Vilas-Boas, M.; Barros, L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem., 2007, 100, 1511-1516.
[23]
Sithisarn, P.; Jarikasem, S. Antioxidant activity of Acanthopanax trifoliatus. Med. Princ. Pract., 2009, 18, 393-398.
[24]
Bauer, A.W.; Kirby, W.M.M.; Sherris, J.C.; Turck, M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 1966, 45, 493-496.
[25]
CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard—Ninth Edition. CLSI document M07-A9. Clinical and Laboratory Standards Institute, 2012, 32, 1-68.
[26]
Chang, C.; Lin, C. Phytochemical composition, antioxidant activity, and neuroprotective effect of Terminalia chebula retzius extract. Evid. Based Complement. Alternat. Med., 2012, 1-7.
[27]
Dubois, M.; Gilles, K.A.; Hamilton, J.K.; Rebers, P.A.; Smith, F. Colorimetric method for determination of sugars and related substances. Anal. Chem., 1956, 28(3), 350-356.
[28]
Tong, S.Y.; Davis, J.S.; Eichenberger, E.; Holland, T.L.; Fowler, V.G. Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clin. Microbiol. Rev., 2015, 28(3), 603-661.
[29]
Centers for Disease Control and Prevention. Food Safety. Staphylococcal Food Poisoning. Available from: https://www.cdc.gov/ foodsafety/diseases/staphylococcal.html (Accessed March 22, 2018).
[30]
Rasmussen, R.V.; Fowler, V.G.; Skov, R.; Bruun, N.E. Future challenges and treatment of Staphylococcus aureus bacteremia with emphasis on MRSA. Future Microbiol., 2011, 6(1), 43-56.
[31]
Smoot, D.T. How does Helicobacter pylori cause mucosal damage? Direct mechanisms. Gastroenterology, 1997, 113(6)(Suppl.), S31-S34.
[32]
Lee, J.H.; Lee, S.J.; Choi, Y.H.; Chung, K.T.; Jeong, Y.K.; Choi, B.T. Effects of mycelial culture of Phellinus linteus on ethanol-induced gastric ulcer in rats. Phytother. Res., 2006, 20, 396-402.
[33]
Mo, S.; Wang, S.; Zhou, G.; Yang, Y.; Li, Y.; Chen, X.; Shi, J. Phelligridins C−F: Cytotoxic pyrano[4,3-c][2]benzopyran-1,6-dione and furo[3,2-c]pyran-4-one derivatives from the fungus Phellinus igniarius. J. Nat. Prod., 2004, 67(5), 823-828.
[34]
Kakkar, S.; Bais, S. A review on protocatechuic acid and its pharmacological potential. ISRN Pharmacol., 2014, 1-9.
[35]
Lee, Y.S.; Kang, Y.H.; Jung, J.Y.; Kang, I.J.; Han, S.N.; Chung, J.S.; Shin, H.K.; Lim, S.S. Inhibitory constituents of aldose reductase in the fruiting body of Phellinus linteus. Biol. Pharm. Bull., 2008, 31(4), 765-768.
[36]
Lee, Y.S.; Kang, Y.H.; Jung, J.Y.; Lee, S.; Ohuchi, K.; Shin, H.K.; Kang, I.J.; Park, J.H.Y.; Shin, H.K.; Lim, S.S. Protein glycation inhibitors from the fruiting body of Phellinus linteus. Biol. Pharm. Bull., 2008, 31(10), 1968-1972.
[37]
Lim, Y.; Lee, S.; Kim, J.; Shin, J.; Kwon, O. A Phellinus baumii-based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathways in vitro and in vivo. J. Funct. Foods, 2016, 24, 231-243.
[38]
Herrmann, K. Occurrence and content of hydroxycinnamic and hydroxybenzoic acid compounds in foods. Crit. Rev. Food Sci. Nutr., 1989, 28(4), 315-347.
[39]
Kayano, S.; Kikuzaki, H.; Fukutsuka, N.; Mitani, T.; Nakatani, N. Antioxidant activity of prune (Prunus domestica L.) constituents and a new synergist. J. Agric. Food Chem., 2002, 50, 3708-3712.
[40]
Sang, S.; Lapsley, K.; Jeong, W.; Lachance, P.A.; Ho, C.; Rosen, R.T. Antioxidative phenolic compounds isolated from almond skins (Prunus amygdalus Batsch). J. Agric. Food Chem., 2002, 50, 2459-2463.
[41]
Li, X.; Wang, X.; Chen, D.; Chen, S. Antioxidant activity and mechanism of protocatechuic acid in vitro. Funct. Food Health Dis., 2011, 7, 232-244.
[42]
Pacheco-Palencia, L.A.; Mertens-Talcott, S.; Talcott, S.T. Chemical composition, antioxidant properties, and thermal stability of a phytochemical enriched oil from Acai (Euterpe oleracea Mart.). J. Agric. Food Chem., 2008, 56, 4631-4636.
[43]
Hudson, E.A.; Dinh, P.A.; Kokubun, T.; Simmonds, M.S.J.; Gescher, A. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiol. Biomarkers Prev., 2000, 9(11), 1163-1170.
[44]
Wikipedia The free encyclopedia. Protocatechuic acid. Available from: https://en.wikipedia.org/wiki/Protocatechuic_acid (Accessed March 22, 2018)
[45]
Link, K.P.; Angell, H.R.; Walker, J.C. The isolation of protocatechuic acid from pigmented onion scales and its significance in relation to disease resistance in onions. J. Biol. Chem., 1929, 81, 369-375.
[46]
Chao, C.; Yin, M. Antibacterial effects of roselle calyx extracts and protocatechuic acid in ground beef and apple juice. Foodborne Pathog. Dis., 2009, 6, 201-206.
[47]
Hassan, H.S.; Musa, A.M.; Usman, M.A.; Abdulaziz, M. Preliminary phytochemical and antispasmodic studies of the stem bark of Boswellia dalzielii. Niger. J. Pharm. Sci., 2009, 8, 1-6.
[48]
Liu, C.; Wang, J.; Chu, C.; Cheng, M.; Tseng, T. In vivo protective effect of protocatechuic acid on tert-butyl hydroperoxide-induced rat hepatotoxicity. Food Chem. Toxicol., 2002, 40, 635-641.
[49]
Jaijoy, K.; Soonthornchareonnon, N.; Panthong, A.; Sireeratawong, S. Anti-inflammatory and analgesic activities of the water extract from the fruit of Phyllanthus emblica Linn. Int. J. Appl. Res. Nat. Prod., 2010, 3, 28-35.
[50]
Zhou, L.; Zuo, Z.; Chow, M.S. Danshen: An overview of its chemistry, pharmacology, pharmacokinetics, and clinical use. J. Clin. Pharmacol., 2005, 45, 1345-1359.
[51]
Zhou, R.; He, L.F.; Li, Y.J.; Shen, Y.; Chao, R.B.; Du, J.R. Cardioprotective effect of water and ethanol extract of Salvia miltiorrhiza in an experimental model of myocardial infarction. J. Ethnopharmacol., 2012, 139, 440-446.
[52]
Guan, S.; Bao, Y.; Jiang, B.J.; An, L. Protective effect of protocatechuic acid from Alpinia oxyphylla on hydrogen peroxide-induced oxidative PC12 cell death. Eur. J. Pharmacol., 2006, 538, 73-79.
[53]
Mo, S.Y.; Yang, Y.C.; Shi, J.G. Studies on chemical constitutes of Phellinus igniarius. Chin. J. Chin. Mater. Med., 2003, 28(4), 339-341.

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