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Current Drug Discovery Technologies


ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

Research Article

β-cell Regenerative Potential of Selected Herbal Extracts in Alloxan Induced Diabetic Rats

Author(s): Anoja Priyadarshani Attanayake*, Kamani Ayoma Perera Wijewardana Jayatilaka, Lakmini Kumari Boralugoda Mudduwa and Chitra Pathirana

Volume 16, Issue 3, 2019

Page: [278 - 284] Pages: 7

DOI: 10.2174/1570163815666180418153024

Price: $65


Background: Effective β-cell regeneration is a recognized therapeutic strategy in the treatment of type 1 diabetes mellitus. Regeneration of β-cells could be achieved via exogenous natural sources as medicinal plant extracts. Medicinal plants selected for the investigation were Spondias pinnata (Linn. f.) Kurz, Coccinia grandis (L.) Voigt and Gmelina arborea Roxb. The objective was to determine the β-cell regenerative potential of these plant extracts in alloxan-induced diabetic rats. Alloxan monohydrate was used to induce diabetes (150 mg/kg, ip).

Methods: Wistar albino rats were divided into six groups (n=6); healthy untreated rats (healthy control), alloxan-induced diabetic untreated rats (diabetic control), diabetic rats received the extracts (treatment groups) of S. pinnata (1.0 g/kg), C. grandis (0.75 g/kg), G. arobrea (1.00 g/kg) and diabetic rats received glibenclamide (0.5 mg/kg; positive control). The above treatment was continued for 30 days. On the 30th day, the rats were sacrificed and biochemical parameters were determined. In addition, histopathology and immunohistochemistry on the pancreatic tissue were done on the 30th day.

Results: According to the results obtained for biochemical parameters, there was a significant increase in the concentrations of serum insulin and C-peptide in plant extracts treated diabetic rats (p < 0.05). The extract of C. grandis produced the highest degree of β-cell regeneration demonstrated through an increase in the number of islets and percentage of the insulin-secreting β-cells (75%) in the pancreas of diabetic rats (p < 0.05) based on the histopathology and immunohistochemistry findings.

Conclusion: The results revealed that the selected extracts of C. grandis (0.75 g/kg), G. arborea (1.00 g/kg) and S. pinnata (1.00 g/kg) exerted β-cell regenerative potential in diabetic rats. The three plant extracts would be valued as natural agents of prompting the β-cell regeneration in vivo.

Keywords: β-cell, Coccinia grandis, Gmelina arborea, insulin-secreting cells, Spondias pinnata, Sri Lankan medicinal plant extracts.

Graphical Abstract
Akpaso MI, Atangwho IJ, Akpantah A, Fischer VA, Igiri AO, Ebong PE. Effect of combined leaf extracts of Vernonia amygdalina (Bitter Leaf) and Gongronema latifolium (Utazi) on the pancreatic β-cells of streptozotocin-induced diabetic rats. Br J Med Res 2011; 1: 24-34.
Ashcroft FM, Rorsman P. Diabetes mellitus and the β cell: The last ten years. Cell 2012; 148: 1160-71.
Ferrannini E. The stunned beta cell: A brief history. Cell Metab 2010; 11(5): 349-52.
Vetere A, Choudhary A, Burns SM, Wagner BK. Targeting the pancreatic β-cell to treat diabetes. Nat Rev Drug Discov 2014; 13: 278-89.
Hosseini A, Shafiee-Nick R, Ghorbani A. Pancreatic beta cell protection/regeneration with phytotherapy. Braz J Pharm Sci 2015; 51: 1-16.
Coman C, Rugina OD, Socaciu C. Plants and natural compounds with antidiabetic action. Not Bot Horti Agrobo 2012; 40(1): 314-25.
Ediriweera ERHSS, Ratnasooriya WD. A review on herbs used in treatment of diabetes mellitus by Sri Lankan Ayurvedic and traditional physicians. Ayu 2009; 30(4): 373-91.
Jayaweera DMA. Medicinal Plants (indigenous and exotic) used in Ceylon. Sri Lanka: National Science Foundation in Sri Lanka 1982.
Tiwari P, Ahmad K, Baig MH. Gymnema sylvestre for diabetes: From traditional herb to future’s therapeutic. Curr Pharm Des 2017; 23(11): 1667-76.
Attanayake AP, Jayatilaka KAPW, Pathirana C, Mudduwa LKB. Phytochemical screening and in vitro antioxidant potentials of extracts of ten medicinal plants used for the treatment of diabetes mellitus in Sri Lanka. Afr J Tradit Complement Altern Med 2015; 12(4): 28-33.
Attanayake AP, Jayatilaka KAPW, Pathirana C, Mudduwa LKB. Antioxidant activity of Gmelina arborea Roxb. (Verbenaceae) bark extract: In vivo and in vitro study. J Med Nutr Nutraceut 2015; 4(1): 32-8.
Attanayake AP, Jayatilaka KAPW, Pathirana C, Mudduwa LKB. Potential antioxidant activities of Spondias pinnata (family: Anacardiaceae) bark extract in rats with streptozotocin induced diabetes mellitus. Int J Pharmacogn 2015; 2: 166-72.
Attanayake AP, Jayatilaka KAPW, Pathirana C, Mudduwa LKB. Study of antihyperglycaemic activity of some Sri Lankan medicinal plants in alloxan induced diabetic rats. Anc Sci Life 2013; 32(4): 193-8.
Ahmed MF, Kazim SM, Ghori SS, et al. Antidiabetic activity of Vinca rosea extracts in alloxan-induced diabetic rats. Int J Endocrinol 2010; 2010841090
Vasconcelos CF, Maranhao HM, Batista TM, Carneiro EM, Ferreira F, Costa J. Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats. J Ethnopharmacol 2011; 137(3): 1533-41.
Abraham EC, Huff TA, Cope ND, Wilson JB Jr, Bransome ED, Jr Huisman TH. Determination of the glycosylated haemoglobins (HbA1) with a new micro column procedure. Suitability of the technique for assessing the clinical management of diabetes mellitus. Diabetes 1978; 27: 931-7.
Johnson RN, Metcalf PA, Baker JR. Fructosamine: A new approach to the estimation of serum glycosylprotein. An index of diabetic control. Clin Chim Acta 1982; 127(1): 87-95.
Andersen L, Dinesen B, Jorgensen PN, Poulsen F, Roder ME. Enzyme immunoassay for intact human insulin in serum or plasma. Clin Chem 1993; 39(4): 578-82.
Ashby JP, Frier BM. Circulating C-peptide: Measurement and clinical application. Ann Clin Biochem 1981; 18: 125-30.
Li QG, Sun R, Gao FZ. Effect of Shen Di Jiang Tang granules on diabetic rats. China J Chin Mater Med 2001; 26: 488-90.
Mac Gregor RR, Williams SJ, Tong PY, Kover K, Moore WV, Stehno-Bittel L. Small rat islets are superior to large islets in in vitro function and in transplantation outcomes. Am J Physiol Endocrinol Metab 2006; 290(5): 771-9.
Elayat AA, El-Naggar MM, Tahir M. An immunocytochemical and morphometric study of the rat pancreatic islets. J Anat 1995; 186: 629-37.
Ghorbani A. Best herbs for managing diabetes: A review of clinical studies. Braz J Pharm Sci 2013; 49(3): 413-22.
Sakurai K, Ogiso T. Effect of ferritin on lambda DNA strand breaks in the reaction system of alloxan plus NADPH cytochrome P450 reductase: Ferritin’s role in diabetogenic action of alloxan. Biol Pharm Bull 1995; 18(2): 262-6.
Adeyemi DO, Komolafe OA, Adewole OS, Obuotor EM, Abiodun AA, Adenowo TK. Histomorphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extracts of Annona muricata. Folia Morphol 2010; 69(2): 92-100.
Aralelimath VR, Bhise SB. Anti-diabetic effects of Gymnema sylvester extract on streptozotocin induced diabetic rats and possible β-cell protective and regenerative evaluations. Dig J Nanomater Biostruct 2012; 7(1): 135-42.
ADA-American Diabetes Association. Standards of medical care in diabetes. Diabetes Care 2013; 36: 11-66.
Borgohain R, Lahon K, Das S, Gohain K. Evaluation of mechanism of anti-diabetic activity of Terminalia chebula on alloxan and adrenaline induced diabetic albino rats. IJPBS 2012; 3: 256-66.
Salgado JM, Mansi DN, Gagliardi A. Cissus sicyoides: Analysis of glycaemic control in diabetic rats through biomarkers. J Med Food 2009; 12(4): 722-7.
Marca MC, Loste A, Ramos JJ. Effect of acute hyperglycaemia on the serum fructosamine and blood glycated haemoglobin concentrations in canine samples. Vet Res Commun 2000; 24(1): 11-6.
Ackermann AM, Gannon M. Molecular regulation of pancreatic beta-cell mass development, maintenance and expansion. J Mol Endocrinol 2007; 38(1-2): 193-206.
Mellado-Gil JM, Cobo-Vuilleumier N, Gauthier BR. Islet β-cell mass preservation and regeneration in diabetes mellitus: Four factors with potential therapeutic interest. J Transplant 2012; 2012230870
Juarez-Rojop IE, Diaz-Zagoya JC, Ble-Castillo JL, et al. Hypoglycaemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats. BMC Complement Altern Med 2012; 12: 236.
Nagappa AN, Thakurdesai PA, Venkat-Rao N, Singh J. Antidiabetic activity of Terminalia catappa Linn fruits. J Ethnopharmacol 2003; 88(1): 45-50.
Bouwens l. Rooman I. Regulation of pancreatic beta-cell mass. Physiol Rev 2005; 85(4): 1255-70.
Avolio F, Pfeifer A, Courtney M, et al. From pancreas morphogenesis to β-cell regeneration. Curr Top Dev Biol 2013; 106: 217-38.
Ansarullah BharuchaB, Umarani M, Dwivedi M, Laddha NC, Begum R. Oreocnide integrifolia flavonoids augment reprogramming for islet neogenesis and β-cell regeneration in pancreatectomized BALB/cMice. J Evid Based Complementary Altern Med 2012; 2012260467
Babujanarthanam R, Kavitha P, Pandian MR. Quercitrin, a bioflavonoid improves glucose homeostasis in streptozotocin-induced diabetic tissues by altering glycolytic and gluconeogenic enzymes. Fundam Clin Pharmacol 2010; 24(3): 357-64.

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