Abstract
In this review, I have mainly described the cell cycle inhibitors isolated from microbial metabolites. Once the molecular target of the inhibitor is determined, the inhibitor can be used as bioprobe to dissect the diverse aspect of biological functions in chemical biology research. Reveromycin A and phosmidosine inhibited the protein synthesis of mammalian cells and arrested the cell cycle at G1 phase. Lucilactaene arrested cells at G1 phase through restoration of mutant p53. Tryprostatin A inhibited the microtubule polymerization by interfering with the interaction between tubulin and microtubule associating protein. On the contrary, cyclotryprostatin D, structurally related to tryprostatin A, enhanced the tubulin polymerization. Terpendole E inhibited the motor activity of mitotic kinesin, Eg5 and induced monoastral spindle in M phase.
Keywords: chemical biology, Ilers, p53, cyclin-dependent kinase, tubulin, kinesin, mitosis
Current Medicinal Chemistry
Title: Development and Application of Bioprobes for Mammalian Cell Cycle Analyses
Volume: 10 Issue: 9
Author(s): Hiroyuki Osada
Affiliation:
Keywords: chemical biology, Ilers, p53, cyclin-dependent kinase, tubulin, kinesin, mitosis
Abstract: In this review, I have mainly described the cell cycle inhibitors isolated from microbial metabolites. Once the molecular target of the inhibitor is determined, the inhibitor can be used as bioprobe to dissect the diverse aspect of biological functions in chemical biology research. Reveromycin A and phosmidosine inhibited the protein synthesis of mammalian cells and arrested the cell cycle at G1 phase. Lucilactaene arrested cells at G1 phase through restoration of mutant p53. Tryprostatin A inhibited the microtubule polymerization by interfering with the interaction between tubulin and microtubule associating protein. On the contrary, cyclotryprostatin D, structurally related to tryprostatin A, enhanced the tubulin polymerization. Terpendole E inhibited the motor activity of mitotic kinesin, Eg5 and induced monoastral spindle in M phase.
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Cite this article as:
Osada Hiroyuki, Development and Application of Bioprobes for Mammalian Cell Cycle Analyses, Current Medicinal Chemistry 2003; 10 (9) . https://dx.doi.org/10.2174/0929867033457836
DOI https://dx.doi.org/10.2174/0929867033457836 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
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