摘要
由定期排列的间节回文重复序列(Crispr)及其相关蛋白(Cas)介导的基因组编辑被认为是高效、快速和定点的。生物重要细胞类型和整个生物体内源性基因修饰的工具。通过指定一个2,它已经成为基因组工程中可预测和精确的选择方法。它的引导RNA中的0-nt靶向序列。本文首先对CRISPR系统的生物学进行了综述。接下来,介绍CRISPR-Cas9在各种方法中的应用,如高效生成广泛的生物医学上重要的细胞模型,以及动物的细胞,修饰表基因组,进行全基因组的筛选,基因治疗,在活细胞中标记特定的基因组基因座综述了酵母和细菌的代谢工程和该系统的改变对内源性基因表达的调节作用。
关键词: CRISPR
Current Gene Therapy
Title:CRISPR-cas System as a Genome Engineering Platform: Applications in Biomedicine and Biotechnology
Volume: 18 Issue: 2
关键词: CRISPR
摘要: Genome editing mediated by Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and its associated proteins (Cas) has recently been considered to be used as efficient, rapid and site-specific tool in the modification of endogenous genes in biomedically important cell types and whole organisms. It has become a predictable and precise method of choice for genome engineering by specifying a 20-nt targeting sequence within its guide RNA. Firstly, this review aims to describe the biology of CRISPR system. Next, the applications of CRISPR–Cas9 in various ways, such as efficient generation of a wide variety of biomedically important cellular models as well as those of animals, modifying epigenomes, conducting genome-wide screens, gene therapy, labelling specific genomic loci in living cells, metabolic engineering of yeast and bacteria and endogenous gene expression regulation by an altered version of this system were reviewed.
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CRISPR-cas System as a Genome Engineering Platform: Applications in Biomedicine and Biotechnology, Current Gene Therapy 2018; 18 (2) . https://dx.doi.org/10.2174/1566523218666180221110627
DOI https://dx.doi.org/10.2174/1566523218666180221110627 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
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