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Letters in Organic Chemistry


ISSN (Print): 1570-1786
ISSN (Online): 1875-6255

Research Article

Improved Scalable Synthesis and Biological Activity of “Indoxyl-gal,” a Chromogenic Histochemical Used for the Identification of Lac+ Organisms

Author(s): Brent Banasik*, Tam Mai and Mansour Samadpour

Volume 15 , Issue 10 , 2018

Page: [822 - 825] Pages: 4

DOI: 10.2174/1570178615666180212152030

Price: $65


An improved and operationally simple synthesis of 3-indoxyl-β-D-galactopyranoside (indoxyl- gal) is disclosed. Indoxyl-gal is a sensitive chromogenic histochemical used for the identification of β-galactosidase enzymatic activity (lacZ gene). Synthesis of indoxyl-gal is improved to 25% overall yield in 5 linear steps from affordable starting materials with minimal chromatography. Biological testing confirmed the detection of β-galactosidase activity in all (100%, 19 strains) coliform bacteria using membrane filtration and modified MI agar.

Keywords: 3-Indoxyl-β-D-galactopyranoside, β-galactosidase, chromogenic dye, coliform bacteria, histochemistry, indicators.

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