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Current Organic Chemistry

Editor-in-Chief

ISSN (Print): 1385-2728
ISSN (Online): 1875-5348

Review Article

Recent Advances of Butelase 1 in Peptide/Protein Ligations

Author(s): Ye Guo*, Jia-Xing Wang, Hong-Li Zhang and Xiao-Wen Fan

Volume 22 , Issue 8 , 2018

Page: [780 - 788] Pages: 9

DOI: 10.2174/1385272822666180122152951

Price: $65

Abstract

Chemoselective ligation reactions provide facile access to various peptides and proteins including cyclic peptides, posttranslationally modified proteins, and peptide/ protein probes. Recently, peptide-ligase-based chemo-enzymatic methods have emerged as promising alternatives to chemical approaches. Ligation mediated by the newly discovered butelase 1 represents one of the state-of-the-art chemo-enzymatic strategies for its high catalytic efficiency and unique substrate specificity. The butelasemediated ligation can be carried out in a semisynthetic manner, in which chemically synthesized peptides and expressed proteins are used as substrates, rendering it suitable for various applications including site-specific modification of proteins and the preparation of thioesters. In this review, we summarize the recent studies and applications of butelase 1.

Keywords: Chemoselective ligation reaction, peptide ligase, butelase 1, peptide/protein drugs, cyclic peptides, protein modification.

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