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Research Article

互补色谱-质谱联用分析泌尿生殖道肿瘤的代谢异质性

卷 26, 期 1, 2019

页: [216 - 231] 页: 16

弟呕挨: 10.2174/0929867324666171006150326

open access plus

摘要

背景：在泌尿生殖道癌症研究方面，预计2012年美国将有340650例新病例和58360例因生殖系统癌症死亡，约141140例新病例和29330例因泌尿系统死亡。现有诊断试验的主要缺点是特异性低、成本高、侵入性强。 目的：本试验研究的主要目的是测定和比较泌尿生殖道癌症患者和健康对照者的尿代谢指印。 方法：采用LC-TOF/MS和GCQQ/MS对30例泌尿生殖系统（膀胱（n=10）、肾脏（n=10）、前列腺（n=10））癌患者和30例健康志愿者的尿液代谢情况进行比较分析，采用U-Mann-Whitney检验或Student t t t检验进行数据分析。主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）。 结果：与健康组相比，膀胱癌、前列腺癌和肾癌中的33、43和22种化合物具有统计学意义。 结论：鉴定了嘌呤、糖、氨基酸、核苷、有机酸等在嘌呤代谢、三羧酸循环、氨基酸代谢或肠道微生物群代谢中起作用的多种化合物。研究发现，三种癌症中只有两种常见的代谢产物，即咖啡酸和乳酸。

关键词: LC-MS，GC-MS，代谢组学，膀胱癌，肾癌，前列腺癌。

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