Abstract
Whereas the majority of microarray applications still deal with expression analysis for gathering information about levels of gene products at certain cell states, other approaches simply ask the question whether particular genes, which are usually indicative for particular microorganisms and pathogens, are present in a sample or not. Investigations that are more detailed try to evaluate the presence of particular subtypes of a given pathogen. The combination of microarray technology and virus diagnostics promises to generate an ideal platform for fast, sensitive, specific, and parallelized virus diagnostics. Performing virus diagnostics on microarrays, however, requires other basic techniques to be optimized. This is necessary in order to obtain unambiguous and reproducible results, which are compatible with the needs for clinical routine. Parameters that have to be considered include supports, coupling chemistry, chemical oligonucleotide synthesis, signal enhancement strategies, and optimal coordination of PCR reactions, hybridizations, and signal detection, as well as interpretation strategies. Finally, considerations should be given to economic aspects, one chipone patient strategies and low integrated arrays as a custom-tailored way to fast and accurate diagnostic tools.
Keywords: virus diagnostics, dna microarrays, on-chip synthesis, chemical oligonucleotide synthesis, dna immobilization chemistry, dendrimers, probe specificity, fluorescence detection
Current Pharmaceutical Biotechnology
Title: Virus Diagnostics on Microarrays
Volume: 4 Issue: 6
Author(s): Hans-Martin Striebel, Eckhard Birch-Hirschfeld, Renate Egerer and Zeno Foldes-Papp
Affiliation:
Keywords: virus diagnostics, dna microarrays, on-chip synthesis, chemical oligonucleotide synthesis, dna immobilization chemistry, dendrimers, probe specificity, fluorescence detection
Abstract: Whereas the majority of microarray applications still deal with expression analysis for gathering information about levels of gene products at certain cell states, other approaches simply ask the question whether particular genes, which are usually indicative for particular microorganisms and pathogens, are present in a sample or not. Investigations that are more detailed try to evaluate the presence of particular subtypes of a given pathogen. The combination of microarray technology and virus diagnostics promises to generate an ideal platform for fast, sensitive, specific, and parallelized virus diagnostics. Performing virus diagnostics on microarrays, however, requires other basic techniques to be optimized. This is necessary in order to obtain unambiguous and reproducible results, which are compatible with the needs for clinical routine. Parameters that have to be considered include supports, coupling chemistry, chemical oligonucleotide synthesis, signal enhancement strategies, and optimal coordination of PCR reactions, hybridizations, and signal detection, as well as interpretation strategies. Finally, considerations should be given to economic aspects, one chipone patient strategies and low integrated arrays as a custom-tailored way to fast and accurate diagnostic tools.
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Striebel Hans-Martin, Birch-Hirschfeld Eckhard, Egerer Renate and Foldes-Papp Zeno, Virus Diagnostics on Microarrays, Current Pharmaceutical Biotechnology 2003; 4 (6) . https://dx.doi.org/10.2174/1389201033377274
DOI https://dx.doi.org/10.2174/1389201033377274 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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