Gold Nanoparticles Conjugated Levofloxacin: For Improved Antibacterial Activity Over Levofloxacin Alone

Title:Gold Nanoparticles Conjugated Levofloxacin: For Improved Antibacterial Activity Over Levofloxacin Alone

Volume: 14 Issue: 8

Author(s): Paramdeep Bagga, Hifazat Hussain Siddiqui, Juber Akhtar, Tariq Mahmood, Manaal Zahera and Mohd Sajid Khan*

Affiliation:

• Department of Biosciences, Integral University, Lucknow 226026,India

Keywords: Gold nanoparticles, human serum albumin, levofloxacin, nitrate reductase, zeta potential, antibiotics.

Abstract: Background: Levofloxacin is a potent antibiotic with severe side effects due to its high doses. Bacterial resistance may be due to frequent use of antibiotics. Biogenic gold nanoparticles conjugated levofloxacin (Au-HSA-LvN-NPs) were developed by Human Serum Albumin (HSA) and nitrate reductasemediated pathways.

Methods: Au-HSA-LvN-NPs (size = 27.2 ± 1 nm) were readily generated with high emulsion stability zeta potential (-13.3 mV). The developed nanoparticles were also characterized by UVvisible spectroscopy, Transmission Electron Microscopy and Dynamic Light Scattering techniques.

Results: The optimized nanoparticles were found efficient against both Gram-positive bacteria and Gramnegative bacteria specifically S. aureus (MIC-0.373 µg/ml), E. coli (MIC-0.149 µg/ml) and P. aeruginosa (MIC-0.346 µg/ml) respectively.

Conclusion: The efficiency of bioconjugated levofloxacin got improved by 1.94 times, 2.89 times and 1.46 times against S. aureus, E. coli and P. aeruginosa respectively, in comparison to pure levofloxacin.

Cite this article as:

Bagga Paramdeep, Siddiqui Hussain Hifazat , Akhtar Juber, Mahmood Tariq , Zahera Manaal and Khan Sajid Mohd *, Gold Nanoparticles Conjugated Levofloxacin: For Improved Antibacterial Activity Over Levofloxacin Alone, Current Drug Delivery 2017; 14 (8) . https://dx.doi.org/10.2174/1567201814666170316113432