Differentiation Potential of Mesenchymal Stem Cells Derived from Adipose Tissue vs Bone Marrow Toward Annulus Fibrosus Cells In vitro

Title:Differentiation Potential of Mesenchymal Stem Cells Derived from Adipose Tissue vs Bone Marrow Toward Annulus Fibrosus Cells In vitro

Volume: 12 Issue: 5

Author(s): Yifei Zhou, Xuqi Hu, Xuhao Zheng, Yaosen Wu, Naifeng Tian, Huazi Xu*Xiaolei Zhang*

Affiliation:

• Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province,China

• Department of Orthopaedics, The 2nd Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province,China

Keywords: Bone marrow stem cells, adipose-derived stem cells, annulus fibrosus cells, coculture, degenerative disc disease.

Abstract: Background: Bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) are two types of stem cells that commonly used in exogenous tissue engineering as well as endogenous tissue repair, however which type of cell is superior remains unknown.

Objective: This study was conducted to compare differentiation potential of BMSCs and ADSCs from Sprague-Dawley rats towards annulus fibrosus (AF)-like cells. Method: Stem cells and AF cells were cocultured with direct cell-to-cell contact in coculture plates with 0.4μ m pore size up till 21 days. AF markers expression at the histological level and molecular level were evaluated by histological analysis and dimethplmethplene blue (DMMB) assay as well as quantitative reverse-transcription polymerase chain reaction (qRT-PCR).

Results: qRT-PCR) results showed up regulation in AF marker gene expressions of both two MSCs after coculture. BMSCs demonstrated a statistically increased expression of collagen II and aggrecan from 7 days of coculture. However, these gene expressions in ADSCs showed a statistically increase at day 14 and day 21. And there was a statistically increase of Collagen I expression in BMSCs at 14 days and 21 days. But only after 21 days of coculture, ADSCs showed a statistically increase in collagen I expression. Moreover, histological analysis and dimethplmethplene blue (DMMB) assay mirrored the results of qRT-PCR. Morphologically, BMSCs became enlarged and triangular, while ADSCs were enlarged and rounded after coculture.

Conclusion: These findings suggested that BMSCs might be superior as a cell source for annulus fibrosus repair compared to ADSCs.

Cite this article as:

Zhou Yifei, Hu Xuqi, Zheng Xuhao, Wu Yaosen, Tian Naifeng, Xu Huazi*, Zhang Xiaolei*, Differentiation Potential of Mesenchymal Stem Cells Derived from Adipose Tissue vs Bone Marrow Toward Annulus Fibrosus Cells In vitro, Current Stem Cell Research & Therapy 2017; 12 (5) . https://dx.doi.org/10.2174/1574888X12666170214093955

DOI https://dx.doi.org/10.2174/1574888X12666170214093955	Print ISSN 1574-888X
Publisher Name Bentham Science Publisher	Online ISSN 2212-3946