Abstract
Background: A reversed-phase high-performance liquid chromatographic method is developed for quantitative analysis of methylprednisolone sodium succinate (MPSS) in nanoparticles.
Method: An ACE 5AQ column (4.6 × 210 mm, 5 µm, Advanced Chromatography Technologies) was used as stationary phase and phosphate buffered saline (PBS, pH 7.4): acetonitrile (70:30 v/v) at a flowrate of 1.5 mL min-1 was used as mobile phase. The retention time of MPSS was around 5 min. The UV detection was performed at 243 nm. The proposed method was precise, accurate, and linear over the concentration range of analysis (1-100 µg mL-1, R2=0.9999) with a limit of detection and a limit of quantification of 0.03 and 0.12 µg mL-1, respectively. Result: No interference was observed from the nanoparticle formulation ingredients. The method validated was applied for the analysis of MPSS in-vitro release samples of polycaprolactone (PCL) nanoparticles.Keywords: Methylprednisolone sodium succinate, HPLC, validation, nanoparticles, pharmaceuticals.
Current Pharmaceutical Analysis
Title:An Improved and Validated HPLC Method for the Determination of Methylprednisolone Sodium Succinate and its Degradation Products in Nanoparticles
Volume: 13 Issue: 2
Author(s): Yasemin Karabey-Akyurek, Emirhan Nemutlu, Erem Bilensoy and Levent Oner
Affiliation:
Keywords: Methylprednisolone sodium succinate, HPLC, validation, nanoparticles, pharmaceuticals.
Abstract: Background: A reversed-phase high-performance liquid chromatographic method is developed for quantitative analysis of methylprednisolone sodium succinate (MPSS) in nanoparticles.
Method: An ACE 5AQ column (4.6 × 210 mm, 5 µm, Advanced Chromatography Technologies) was used as stationary phase and phosphate buffered saline (PBS, pH 7.4): acetonitrile (70:30 v/v) at a flowrate of 1.5 mL min-1 was used as mobile phase. The retention time of MPSS was around 5 min. The UV detection was performed at 243 nm. The proposed method was precise, accurate, and linear over the concentration range of analysis (1-100 µg mL-1, R2=0.9999) with a limit of detection and a limit of quantification of 0.03 and 0.12 µg mL-1, respectively. Result: No interference was observed from the nanoparticle formulation ingredients. The method validated was applied for the analysis of MPSS in-vitro release samples of polycaprolactone (PCL) nanoparticles.Export Options
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Cite this article as:
Karabey-Akyurek Yasemin, Nemutlu Emirhan, Bilensoy Erem and Oner Levent, An Improved and Validated HPLC Method for the Determination of Methylprednisolone Sodium Succinate and its Degradation Products in Nanoparticles, Current Pharmaceutical Analysis 2017; 13 (2) . https://dx.doi.org/10.2174/1573412913666161107155008
DOI https://dx.doi.org/10.2174/1573412913666161107155008 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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