Abstract
Background: Recent advances in proteomics methodologies allow for high throughput profiling of proteolytic cleavage events. The resulting substrate peptide distributions provide deep insights in the underlying macromolecular recognition events, as determinants of biomolecular specificity identified by proteomics approaches may be compared to structure-based analysis of corresponding protein-protein interfaces.
Method: Here, we present an overview of experimental and computational methodologies and tools applied in the area and provide an outlook beyond the protein class of proteases. Results and Conclusion: We discuss here future potential, synergies and needs of the emerging overlap disciplines of proteomics and structure-based modelling.Keywords: Macromolecular recognition, molecular modelling, peptide binding, protein-protein-interface, protease substrate profiling, specificity.
Current Protein & Peptide Science
Title:Determinants of Macromolecular Specificity from Proteomics-Derived Peptide Substrate Data
Volume: 18 Issue: 9
Author(s): Julian E. Fuchs*, Oliver Schilling and Klaus R. Liedl
Affiliation:
- Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW,United Kingdom
Keywords: Macromolecular recognition, molecular modelling, peptide binding, protein-protein-interface, protease substrate profiling, specificity.
Abstract: Background: Recent advances in proteomics methodologies allow for high throughput profiling of proteolytic cleavage events. The resulting substrate peptide distributions provide deep insights in the underlying macromolecular recognition events, as determinants of biomolecular specificity identified by proteomics approaches may be compared to structure-based analysis of corresponding protein-protein interfaces.
Method: Here, we present an overview of experimental and computational methodologies and tools applied in the area and provide an outlook beyond the protein class of proteases. Results and Conclusion: We discuss here future potential, synergies and needs of the emerging overlap disciplines of proteomics and structure-based modelling.Export Options
About this article
Cite this article as:
Fuchs E. Julian*, Schilling Oliver and Liedl R. Klaus, Determinants of Macromolecular Specificity from Proteomics-Derived Peptide Substrate Data, Current Protein & Peptide Science 2017; 18 (9) . https://dx.doi.org/10.2174/1389203717666160724211231
DOI https://dx.doi.org/10.2174/1389203717666160724211231 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
Call for Papers in Thematic Issues
Advancements in Proteomic and Peptidomic Approaches in Cancer Immunotherapy: Unveiling the Immune Microenvironment
The scope of this thematic issue centers on the integration of proteomic and peptidomic technologies into the field of cancer immunotherapy, with a particular emphasis on exploring the tumor immune microenvironment. This issue aims to gather contributions that illustrate the application of these advanced methodologies in unveiling the complex interplay ...read more
Nutrition and Metabolism in Musculoskeletal Diseases
The musculoskeletal system consists mainly of cartilage, bone, muscles, tendons, connective tissue and ligaments. Balanced metabolism is of vital importance for the homeostasis of the musculoskeletal system. A series of musculoskeletal diseases (for example, sarcopenia, osteoporosis) are resulted from the dysregulated metabolism of the musculoskeletal system. Furthermore, metabolic diseases (such ...read more
Protein Folding, Aggregation and Liquid-Liquid Phase Separation
Protein folding, misfolding and aggregation remain one of the main problems of interdisciplinary science not only because many questions are still open, but also because they are important from the point of view of practical application. Protein aggregation and formation of fibrillar structures, for example, is a hallmark of a ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Challenge of Exploiting ABCG2 in the Clinic
Current Pharmaceutical Biotechnology The Complexities of TGF-β Action During Mammary and Squamous Cell Carcinogenesis
Current Pharmaceutical Biotechnology Cardiotonic Steroids in Adaptation to Dietary Salt Intake
Current Clinical Pharmacology The Role of Antioxidant Enzymes in the Growth of Pancreatic Carcinoma
Current Cancer Therapy Reviews Selected Approaches for Rational Drug Design and High Throughput Screening to Identify Anti-Cancer Molecules
Anti-Cancer Agents in Medicinal Chemistry Scenario of the Treatment of Arthritis with Natural Products
Recent Patents on Inflammation & Allergy Drug Discovery Hypoxia and Inflammation in Prostate Cancer Progression. Cross-talk with Androgen and Estrogen Receptors and Cancer Stem Cells
Endocrine, Metabolic & Immune Disorders - Drug Targets The Discovery of the Potent and Selective Antitumour Agent 2-(4-Amino-3-methylphenyl)benzothiazole (DF 203) and Related Compounds
Current Medicinal Chemistry Assays for Identification of Hsp90 Inhibitors and Biochemical Methods for Discriminating their Mechanism of Action
Current Topics in Medicinal Chemistry Antioxidant Phytochemicals in Pulses and their Relation to Human Health: A Review
Current Pharmaceutical Design “Self-Nonself” Peptides in the Design of Vaccines
Current Pharmaceutical Design Hepatocyte Growth Factor is a Potential Lymphangiogenic Factor; Clinical Implications
Current Signal Transduction Therapy Disrupting the mTOR Signaling Network as a Potential Strategy for the Enhancement of Cancer Radiotherapy
Current Cancer Drug Targets Novel Indications for Benzodiazepine Antagonist Flumazenil in GABA Mediated Pathological Conditions of the Central Nervous System
Current Pharmaceutical Design Effects of Polymorphisms of the CYP450 Enzyme Genes on Estrogen Status and the Risk for Osteoporosis
Current Pharmacogenomics Biomarkers in Asthma and COPD
Recent Patents on Biomarkers Genetics and Epigenetics of Lung Cancer: Mechanisms and Future Perspectives
Current Cancer Therapy Reviews Gene Therapy Using IL-12 Family Members in Infection, Auto-Immunity, and Cancer
Current Gene Therapy Preparation, Photoluminescence and Antimicrobial Activity of Loracarbef and its Various Complexes
Current Analytical Chemistry Rational Design and Development of RDP58
Current Pharmaceutical Design