Generic placeholder image

Current Biotechnology


ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

Research Article

Improved Hemicellulase Production by Genetic Modification of Carbon Catabolite Repression and Xylanolitic Activation in Aspergillus niger

Author(s): Diogo Robl*, Isabelle Benoit, Gabriel Padilla, Jose Geraldo da Cruz Pradella and Ronald P. de Vries

Volume 7, Issue 1, 2018

Page: [10 - 18] Pages: 9

DOI: 10.2174/2211550105666160722120556

Price: $65


Background: Cultivation and media optimization is one way to improve hemicellulase production in Aspergillus niger, but bioprocess approaches have limitations due to the carbon catabolite repression (CCR) process.

Objective: This study aimed to improve hemicellulase production by genetic modifications at CCR levels in A. niger strain.

Method: Deletion of the creA gene to derepress the expression of the hemicellulolytic genes and introduction of a constitutively active version of xlnR were performed.

Results and Discussion: The deletion of creA resulted in a higher expression of some hemicellulolytic genes and a higher specific enzyme production. Introduction of the constitutively active xlnR in a ΔCreA strain increased fungal biomass production, but did not result in a higher expression/production of most hemicellulases on xylose, although it was possible to double the productivity of β-xylosidase.

Conclusions: A dominant effect of creA deletion on xlnR overexpression in the production of biomass degrading enzymes was present and indicated that a mechanism not totally elucidated is present and involves fungal growth and inductor.

Keywords: Aspergillus niger, carbon catabolic repression, hemicellulases, enzyme production, biomass, plant cell wall degradation.

Graphical Abstract

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy