Abstract
In this paper, we reported the development of a micro-flow label-free impedimetric biosensor based on the use of thin-film interdigitated gold array microelectrodes (IDA) for the detection of carbohydrate antigen 125 (CA125). The immunosensor is developed through the electropolymerization of anthranilic acid (AA) on the surface of IDA electrodes followed by the covalent attachment of anti-CA125 monoclonal antibody. CA125 protein affinity reaction was then evaluated by means of electrochemical impedance spectroscopy (EIS). The sensor was characterized by electrochemical techniques and scanning electron microscopy (SEM). Using the optimized experimental conditions, the developed immunosensor showed a good analytical performance for CA125 detection from 0 to 100 U/mL with estimated limit of detection (LOD = 3Sblank/Slope) of 7 U/mL.
Keywords: Antrhanilic acid, cancer antigen 125, electrochemical impedance spectroscopy, thin-film interdigitated gold array microelectrodes (IDA).
Current Drug Delivery
Title:Micro-flow Immunosensor Based on Thin-film Interdigitated Gold Array Microelectrodes for Cancer Biomarker Detection
Volume: 13 Issue: 3
Author(s): Andrea Ravalli, Luca Lozzi and Giovanna Marrazza
Affiliation:
Keywords: Antrhanilic acid, cancer antigen 125, electrochemical impedance spectroscopy, thin-film interdigitated gold array microelectrodes (IDA).
Abstract: In this paper, we reported the development of a micro-flow label-free impedimetric biosensor based on the use of thin-film interdigitated gold array microelectrodes (IDA) for the detection of carbohydrate antigen 125 (CA125). The immunosensor is developed through the electropolymerization of anthranilic acid (AA) on the surface of IDA electrodes followed by the covalent attachment of anti-CA125 monoclonal antibody. CA125 protein affinity reaction was then evaluated by means of electrochemical impedance spectroscopy (EIS). The sensor was characterized by electrochemical techniques and scanning electron microscopy (SEM). Using the optimized experimental conditions, the developed immunosensor showed a good analytical performance for CA125 detection from 0 to 100 U/mL with estimated limit of detection (LOD = 3Sblank/Slope) of 7 U/mL.
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Cite this article as:
Ravalli Andrea, Lozzi Luca and Marrazza Giovanna, Micro-flow Immunosensor Based on Thin-film Interdigitated Gold Array Microelectrodes for Cancer Biomarker Detection, Current Drug Delivery 2016; 13 (3) . https://dx.doi.org/10.2174/1567201812666151029101217
DOI https://dx.doi.org/10.2174/1567201812666151029101217 |
Print ISSN 1567-2018 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5704 |
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