Abstract
Acetylcholine (ACh) is the main mediator associated with the anti-inflammatory cholinergic pathway. ACh plays an inhibitory role in several inflammatory conditions. Sepsis is a severe clinical syndrome characterized by bacterial dissemination and overproduction of inflammatory mediators. The aim of the current study was to investigate the participation of endogenous ACh in the modulation of inflammatory response induced by a model of polymicrobial sepsis. Wild type (WT) and vesicular acetylcholine transporter knockdown (VAChTKD) mice were exposed to cecal ligation and perforation- induced sepsis. Levels of Tumor Necrosis Factor Alpha (TNF-α) and bacterial growth in peritoneal cavity and serum, and neutrophil recruitment into peritoneal cavity were assessed. The concentration of TNF-α in both compartments was higher in VAChTKD in comparison with WT mice. VAChTKD mice presented elevated burden of bacteria in peritoneum and blood, and impairment of neutrophil migration to peritoneal cavity. This phenotype was reversed by treatment with nicotine salt. These findings suggest that endogenous ACh plays a major role in the control of sepsis-associated inflammatory response.
Keywords: Acetylcholine, nicotine, sepsis, inflammation, neutrophils.
Current Neurovascular Research
Title:Endogenous Acetylcholine Controls the Severity of Polymicrobial Sepsisassociated Inflammatory Response in Mice
Volume: 13 Issue: 1
Author(s): Flávio Almeida Amaral, Caio Tavares Fagundes, Aline Silva Miranda, Vivian Vasconceios Costa, Livia Resende, Danielle da Gloria de Souza, Vania Ferreira Prado, Mauro Martins Teixeira, Marco Antonio Maximo Prado and Antonio Lucio Teixeira
Affiliation:
Keywords: Acetylcholine, nicotine, sepsis, inflammation, neutrophils.
Abstract: Acetylcholine (ACh) is the main mediator associated with the anti-inflammatory cholinergic pathway. ACh plays an inhibitory role in several inflammatory conditions. Sepsis is a severe clinical syndrome characterized by bacterial dissemination and overproduction of inflammatory mediators. The aim of the current study was to investigate the participation of endogenous ACh in the modulation of inflammatory response induced by a model of polymicrobial sepsis. Wild type (WT) and vesicular acetylcholine transporter knockdown (VAChTKD) mice were exposed to cecal ligation and perforation- induced sepsis. Levels of Tumor Necrosis Factor Alpha (TNF-α) and bacterial growth in peritoneal cavity and serum, and neutrophil recruitment into peritoneal cavity were assessed. The concentration of TNF-α in both compartments was higher in VAChTKD in comparison with WT mice. VAChTKD mice presented elevated burden of bacteria in peritoneum and blood, and impairment of neutrophil migration to peritoneal cavity. This phenotype was reversed by treatment with nicotine salt. These findings suggest that endogenous ACh plays a major role in the control of sepsis-associated inflammatory response.
Export Options
About this article
Cite this article as:
Amaral Almeida Flávio, Fagundes Tavares Caio, Miranda Silva Aline, Costa Vasconceios Vivian, Resende Livia, Gloria de Souza da Danielle, Prado Ferreira Vania, Teixeira Martins Mauro, Maximo Prado Antonio Marco and Teixeira Lucio Antonio, Endogenous Acetylcholine Controls the Severity of Polymicrobial Sepsisassociated Inflammatory Response in Mice, Current Neurovascular Research 2016; 13 (1) . https://dx.doi.org/10.2174/1567202612666151026105915
DOI https://dx.doi.org/10.2174/1567202612666151026105915 |
Print ISSN 1567-2026 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5739 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Manipulating Thymic Apoptosis for Future Therapy of Autoimmune Diseases
Current Pharmaceutical Design Novel Agents Targeting Bioactive Sphingolipids for the Treatment of Cancer
Current Medicinal Chemistry Nitrogen, Oxygen or Sulfur Containing Heterocyclic Compounds as Analgesic Drugs Used as Modulators of the Nitroxidative Stress
Mini-Reviews in Medicinal Chemistry Filtering Disturbances in Schizophrenic Patients. Gating of Auditory Evoked Potentials and Prepulse Inhibition of the Acoustic Startle Response Compared. Emphasis on the Role of Dopamine
Current Neuropharmacology Antiinflammatory Activity of Melatonin in Central Nervous System
Current Neuropharmacology PDGF-D Signaling: A Novel Target in Cancer Therapy
Current Drug Targets Challenges in Neuronal Apoptosis
Current Alzheimer Research Lipids at the Cross-road of Autoimmunity in Multiple Sclerosis
Current Medicinal Chemistry Bioinformatic Analysis of HIV-1 Entry and Pathogenesis
Current HIV Research The Past, Present and Future Subclassification of Patients with Acute Myeloid Leukemia
Current Pharmaceutical Biotechnology Drug Delivery Systems for Imaging and Therapy of Parkinson's Disease
Current Neuropharmacology Clinical Efficacy and Effectiveness of Ursodeoxycholic Acid in Cholestatic Liver Diseases
Current Clinical Pharmacology Ectodomain Shedding of ACE and ACE2 as Regulators of Their Protein Functions
Current Enzyme Inhibition Drugs Targeting Parasite Lysosomes
Current Pharmaceutical Design Pharmacological Targeting of Neuronal Kv7.2/3 Channels: A Focus on Chemotypes and Receptor Sites
Current Medicinal Chemistry Progress Towards Liver and Lung-Directed Gene Therapy with Helper- Dependent Adenoviral Vectors
Current Gene Therapy Tumor Growth-Promoting Properties of Macrophage Migration Inhibitory Factor
Current Pharmaceutical Design The Crosstalk between Gut Inflammation and Gastrointestinal Disorders During Acute Pancreatitis
Current Pharmaceutical Design The IL-7/IL-7 Receptor Axis: Understanding its Central Role in T-Cell Homeostasis and the Challenges Facing its Utilization as a Novel Therapy
Current Drug Targets Melphalan in Regional Chemotherapy for Locally Recurrent Metastatic Melanoma
Current Topics in Medicinal Chemistry