摘要
肺的囊肿性纤维化的基因治疗的难点是提供长期的遗传缺陷纠正。这可以通过用整合载体使气道上皮干/祖细胞成为靶点来实现。本文评价了一个慢病毒载体(来源于猴免疫缺陷病毒并用仙台病毒的F和HN包膜蛋白假型化)转导小鼠鼻气道祖基底细胞的能力。我们首先转导了体外富集培养的基底细胞并证实了cytokeratin-5阳性细胞的高效转导。然后我们要问祖细胞是否可以体内转导。我们评估了在小鼠身上用polidocanol鼻腔给药预处理以暴露祖细胞层的转导效率。与对照组小鼠相比,polidocanol治疗的小鼠显示载体给药后3天和14天转导的基底细胞数量显著增加。14天中,被治疗小鼠的上皮组织含有纤毛分化良好的集群(4到8个相邻细胞)和显示克隆扩张的基底细胞。这些结果表明:我们的慢病毒载体可以在体内转导祖基底细胞,虽然转导需要载体给药之前表面上皮组织裸露。
关键词: 囊肿性纤维化,基因治疗,慢病毒,祖基底细胞,Polidocanol。
Current Gene Therapy
Title:Ex Vivo and In Vivo Lentivirus-Mediated Transduction of Airway Epithelial Progenitor Cells
Volume: 15 Issue: 6
Author(s): Giulia Leoni, Marguerite Y. Wasowicz, Mario Chan, Cuixiang Meng, Raymond Farley and Steven L. Brody, Makoto Inoue, Mamoru Hasegawa, Eric W.F.W. Alton and Uta Griesenbach
Affiliation:
关键词: 囊肿性纤维化,基因治疗,慢病毒,祖基底细胞,Polidocanol。
摘要: A key challenge in pulmonary gene therapy for cystic fibrosis is to provide long-term correction of the genetic defect. This may be achievable by targeting airway epithelial stem/progenitor cells with an integrating vector. Here, we evaluated the ability of a lentiviral vector, derived from the simian immunodeficiency virus and pseudotyped with F and HN envelope proteins from Sendai virus, to transduce progenitor basal cells of the mouse nasal airways. We first transduced basal cell-enriched cultures ex vivo and confirmed efficient transduction of cytokeratin-5 positive cells. We next asked whether progenitor cells could be transduced in vivo. We evaluated the transduction efficiency in mice pretreated by intranasal administration of polidocanol to expose the progenitor cell layer. Compared to control mice, polidocanol treated mice demonstrated a significant increase in the number of transduced basal cells at 3 and 14 days post vector administration. At 14 days, the epithelium of treated mice contained clusters (4 to 8 adjacent cells) of well differentiated ciliated, as well as basal cells suggesting a clonal expansion. These results indicate that our lentiviral vector can transduce progenitor basal cells in vivo, although transduction required denudation of the surface epithelium prior to vector administration.
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Giulia Leoni, Marguerite Y. Wasowicz, Mario Chan, Cuixiang Meng, Raymond Farley and Steven L. Brody, Makoto Inoue, Mamoru Hasegawa, Eric W.F.W. Alton and Uta Griesenbach , Ex Vivo and In Vivo Lentivirus-Mediated Transduction of Airway Epithelial Progenitor Cells, Current Gene Therapy 2015; 15 (6) . https://dx.doi.org/10.2174/1566523215666151016123625
DOI https://dx.doi.org/10.2174/1566523215666151016123625 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
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