摘要
将尸体捐赠者的胰岛移植至病人体内可成功地使血糖正常化,这一概念证明1型糖尿病是可治愈的。尽管如此,但要说明的是,这种细胞疗法广泛使用的主要问题为移植后的胰岛细胞缺乏活性及功能不足。基因治疗可在移植前有针对性的提高活性和性能,并提供一个可行的方法来规避上述问题,以维持体内β细胞群功能持久。然而,将核酸安全有效地转移至胰岛仍然是一项具有挑战性的任务。在这里,我们描述一个简单易用的慢病毒载体介导方案,可介导大约80%的老鼠和人胰岛细胞,同时保留胰岛结构,代谢功能和葡萄糖依赖性刺激胰岛素的分泌。我们的方案将促进完全确定整个胰岛细胞异种移植过程中的潜在治疗靶点及基因表达调控。
关键词: 糖尿病,转基因,传染,慢病毒,胰岛,介导
Current Gene Therapy
Title:A Simple High Efficiency Intra-Islet Transduction Protocol Using Lentiviral Vectors
Volume: 15 Issue: 4
Author(s): Carmen Maria Jimenez-Moreno, Irene de Gracia Herrera-Gomez, Livia Lopez-Noriega, Petra Isabel Lorenzo, Nadia Cobo-Vuilleumier, Esther Fuente-Martin, Jose Manuel Mellado-Gil, Geraldine Parnaud, Domenico Bosco and Benoit Raymond Gauthier and Alejandro Martin-Montalvo
Affiliation:
关键词: 糖尿病,转基因,传染,慢病毒,胰岛,介导
摘要: Successful normalization of blood glucose in patients transplanted with pancreatic islets isolated from cadaveric donors established the proof-of-concept that Type 1 Diabetes Mellitus is a curable disease. Nonetheless, major caveats to the widespread use of this cell therapy approach have been the shortage of islets combined with the low viability and functional rates subsequent to transplantation. Gene therapy targeted to enhance survival and performance prior to transplantation could offer a feasible approach to circumvent these issues and sustain a durable functional β-cell mass in vivo. However, efficient and safe delivery of nucleic acids to intact islet remains a challenging task. Here we describe a simple and easy-to-use lentiviral transduction protocol that allows the transduction of approximately 80 % of mouse and human islet cells while preserving islet architecture, metabolic function and glucose-dependent stimulation of insulin secretion. Our protocol will facilitate to fully determine the potential of gene expression modulation of therapeutically promising targets in entire pancreatic islets for xenotransplantation purposes.
Export Options
About this article
Cite this article as:
Carmen Maria Jimenez-Moreno, Irene de Gracia Herrera-Gomez, Livia Lopez-Noriega, Petra Isabel Lorenzo , Nadia Cobo-Vuilleumier, Esther Fuente-Martin, Jose Manuel Mellado-Gil, Geraldine Parnaud, Domenico Bosco and Benoit Raymond Gauthier and Alejandro Martin-Montalvo , A Simple High Efficiency Intra-Islet Transduction Protocol Using Lentiviral Vectors, Current Gene Therapy 2015; 15 (4) . https://dx.doi.org/10.2174/1566523215666150630121557
DOI https://dx.doi.org/10.2174/1566523215666150630121557 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
Call for Papers in Thematic Issues
Programmed Cell Death Genes in Oncology: Pioneering Therapeutic and Diagnostic Frontiers (BMS-CGT-2024-HT-45)
Programmed Cell Death (PCD) is recognized as a pivotal biological mechanism with far-reaching effects in the realm of cancer therapy. This complex process encompasses a variety of cell death modalities, including apoptosis, autophagic cell death, pyroptosis, and ferroptosis, each of which contributes to the intricate landscape of cancer development and ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Chronic Inflammatory Disorders and Accelerated Atherosclerosis: Chronic Kidney Disease
Current Pharmaceutical Design Downregulation of Membrane-bound Angiotensin Converting Enzyme 2 (ACE2) Receptor has a Pivotal Role in COVID-19 Immunopathology
Current Drug Targets n-3 Fatty Acids: Role in Neurogenesis and Neuroplasticity
Current Medicinal Chemistry A Brief Overview of the Oral Delivery of Insulin as an Alternative to the Parenteral Delivery
Current Molecular Medicine A Mendelian Randomization Study on Infant Length and Type 2 Diabetes Mellitus Risk
Current Gene Therapy New-Onset Hyperglycemia and Acute Coronary Syndrome: A Systematic Overview and Meta-Analysis
Current Diabetes Reviews Network Pharmacology of Ayurveda Formulation <i>Triphala</i> with Special Reference to Anti-Cancer Property
Combinatorial Chemistry & High Throughput Screening Nitric Oxide and Oral Diseases: Can We Talk About It?
Cardiovascular & Hematological Agents in Medicinal Chemistry Pharmacological Characteristics of Parenteral IGF-I Administration
Current Pharmaceutical Biotechnology Nitric Oxide: News from Stem Cells to Platelets
Current Medicinal Chemistry Risk Factors for Suicide – An Alternative View
CNS & Neurological Disorders - Drug Targets Current Genetic and Epigenetic Insights into Type 2 Diabetes Mellitus
Endocrine, Metabolic & Immune Disorders - Drug Targets New Chemotherapy and Immunotherapy for Tuberculosis
Current Respiratory Medicine Reviews Angiogenesis, Neurogenesis and Neuroplasticity in Ischemic Stroke
Current Cardiology Reviews Nano- and Biotechnological Approaches in Current and Future Generation of Cardiovascular Stents
Current Nanoscience Structure – Function Relationships of Pre-Fibrillar Protein Assemblies in Alzheimers Disease and Related Disorders
Current Alzheimer Research Lipid Lowering Drugs and Gallstones: A Therapeutic Option?
Current Pharmaceutical Design Challenges for Gene Therapy of Type 1 Diabetes
Current Gene Therapy Intestinal Targeting of Drugs: Rational Design Approaches and Challenges
Current Topics in Medicinal Chemistry An Arylidene-Thiazolidinedione Derivative, GPU-4, without PPARγ Activation, Reduces Retinal Neovascularization
Current Neurovascular Research