Analytical Methods for Determination of Reactive Oxygen Species

Title:Analytical Methods for Determination of Reactive Oxygen Species

Volume: 10 Issue: 4

Author(s): Joanna Pranczk, Dagmara Jacewicz, Dariusz Wyrzykowski and Lech Chmurzynski

Affiliation:

Keywords: Analytical methods, reactive oxygen species, radicals.

Abstract: This review focuses on analytical methods for the determination of reactive oxygen species (ROS): a superoxide/ hydroperoxyl radical, hydrogen peroxide, singlet oxygen and a hydroxyl radical. The methods which are described in this paper are based on: electron paramagnetic resonance spectroscopy (EPR), nuclear magnetic resonance (NMR), mass spectroscopy (MS), spectroscopy UV-Vis, gas chromatography (GC), chemiluminescence (CL), fluorescence (FL), HPLC –UV, iodide titration, and ¹⁴C-formate oxidation. In this review, the most common analytical methods for the detection of ROS generated by chemical methods are presented. The methods are based on the direct as well as indirect detection of ROS.

Cite this article as:

Pranczk Joanna, Jacewicz Dagmara, Wyrzykowski Dariusz and Chmurzynski Lech, Analytical Methods for Determination of Reactive Oxygen Species, Current Pharmaceutical Analysis 2014; 10 (4) . https://dx.doi.org/10.2174/1573412910666140530221254