Effect of Temperature and Solvent on Fibrillation of Human Serum Albumin

Title:Effect of Temperature and Solvent on Fibrillation of Human Serum Albumin

Volume: 22 Issue: 2

Author(s): Nitin K. Pandey, Sudeshna Ghosh, Debi R. Tripathy and Swagata Dasgupta

Affiliation:

• Department of Chemistry, Indian Institute of Technology, Kharagpur, 721302, India.,India

Keywords: Ethanol, fibrillation, human serum albumin, solvent, spectroscopy, temperature.

Abstract: During the past several years, studies on the protein aggregation process in the presence of cosolvents/ co-solutes have been looked into which provides significant insight in the stability of proteins in a crowded cellular milieu. Here, in the present report we have investigated the fibrillation of human serum albumin (HSA) under the mixed aqueous-ethanol solvent conditions at two different temperatures (37 °C and 65 °C). Self-association of protein was monitored using various spectroscopic and microscopic techniques. Results obtained from detailed investigation have shown that fibrillation of human serum albumin is favored at higher temperature (65 °C) at lower ethanol concentration. However, at 37 °C comparatively higher ethanol concentration is the prerequisite condition for fibrillation process to take place.

Cite this article as:

Pandey K. Nitin, Ghosh Sudeshna, Tripathy R. Debi and Dasgupta Swagata, Effect of Temperature and Solvent on Fibrillation of Human Serum Albumin, Protein & Peptide Letters 2015; 22(2) . https://dx.doi.org/10.2174/0929866521666140320104409