Polyphenols are the most abundant antioxidants. Polyphenols are known to non-covalent interact with plasma proteins in blood through hydrophobic or hydrophilic interactions. It was found that the effect of polyphenol-plasma protein interaction (PpPI) on the bioavailability of polyphenols is not equivocal. Because the conclusion of individual reports are contradictory to each other; therefore, it is very difficult to give a univocal comment on the influence of PpPI on antioxidant property of polyphenols. The influence of PpPI on the antioxidant activity of polyphenols is decided by the antioxidant assay, the structure characteristics of polyphenols, as well as the proteins. This mini review mainly focused on the influence of PpPI on the antioxidant properties of polyphenols.