Abstract
Functional expression of recombinant proteins has become a routine, but critical tool in modern molecular biology. Since their introduction, the use of baculovirus vectors to produce proteins for purification has become one of the most widely-used viral gene delivery systems as expression levels obtained are difficult to match with any other eukaryotic expression system. Extensive engineering to simplify and accelerate the process of recombinant virus construction has made this system acessible to virtually any modern biological laboratory. The utility of baculoviruses has been broadened with the discovery that appropriately modified virus can mediate gene expression in a wide variety of mammalian cell lines, and thus can function as a flexible cell-based assay development tool. The wide range of applications and potential for commercialization of products leads to consideration of a number of aspects of the system.
Keywords: DNA, Recombinant viruses, AcMNPV genome, deno-associated virus, mammalian cells
Current Drug Targets
Title: Baculovirus Expression Vectors for Insect and Mammalian Cells
Volume: 8 Issue: 10
Author(s): J. Patrick Condreay and Thomas A. Kost
Affiliation:
Keywords: DNA, Recombinant viruses, AcMNPV genome, deno-associated virus, mammalian cells
Abstract: Functional expression of recombinant proteins has become a routine, but critical tool in modern molecular biology. Since their introduction, the use of baculovirus vectors to produce proteins for purification has become one of the most widely-used viral gene delivery systems as expression levels obtained are difficult to match with any other eukaryotic expression system. Extensive engineering to simplify and accelerate the process of recombinant virus construction has made this system acessible to virtually any modern biological laboratory. The utility of baculoviruses has been broadened with the discovery that appropriately modified virus can mediate gene expression in a wide variety of mammalian cell lines, and thus can function as a flexible cell-based assay development tool. The wide range of applications and potential for commercialization of products leads to consideration of a number of aspects of the system.
Export Options
About this article
Cite this article as:
Condreay Patrick J. and Kost A. Thomas, Baculovirus Expression Vectors for Insect and Mammalian Cells, Current Drug Targets 2007; 8 (10) . https://dx.doi.org/10.2174/138945007782151351
DOI https://dx.doi.org/10.2174/138945007782151351 |
Print ISSN 1389-4501 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5592 |
Call for Papers in Thematic Issues
New drug therapy for eye diseases
Eyesight is one of the most critical senses, accounting for over 80% of our perceptions. Our quality of life might be significantly affected by eye disease, including glaucoma, diabetic retinopathy, dry eye, etc. Although the development of microinvasive ocular surgery reduces surgical complications and improves overall outcomes, medication therapy is ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
A Review of Systemic Vasodilators in Low Cardiac Output Syndrome Following Pediatric Cardiac Surgery
Current Vascular Pharmacology Development of Patient-Specific Hematopoietic Stem and Progenitor Cell Grafts from Pluripotent Stem Cells, In Vitro
Current Molecular Medicine Renin-Angiotensin System in Central Nervous System Diseases and its Interaction with COVID-19
Current Medicinal Chemistry Targeted Delivery for Neurodegenerative Disorders Using Gene Therapy Vectors: Gene Next Therapeutic Goals
Current Gene Therapy Neuromodulation of the Perinatal Respiratory Network
Current Neuropharmacology Troponin in Newborns and Pediatric Patients
Cardiovascular & Hematological Agents in Medicinal Chemistry Current Non-viral Gene Therapy Strategies for the Treatment of Glioblastoma
Current Medicinal Chemistry Robotic Assisted Surgery in Gynecology: Current Insights and Future Perspectives
Recent Patents on Biotechnology Large-Scale Production Means for the Manufacturing of Lentiviral Vectors
Current Gene Therapy Impact of Pulmonary Vascular Resistances in Heart Transplantation for Congenital Heart Disease
Current Cardiology Reviews Prospectives for Gene Therapy of Retinal Degenerations
Current Genomics Fertility Preservation in Women After the Cancer
Current Pharmaceutical Design General Anesthetics in Pediatric Anesthesia: Influences on the Developing Brain
Current Drug Targets Normal Ventricular Functional Reference Parameters on Magnetic Resonance Imaging in Healthy Children
Current Medical Imaging Apis mellifera Proteomics: Where Will the Future Bee?
Current Proteomics Recent Advances in the Transition Metal-Catalyzed Addition of Carboxylic Acids to Alkynes
Current Organic Chemistry RNA-Mediated Therapeutics: From Gene Inactivation to Clinical Application
Current Topics in Medicinal Chemistry Genetics of Congenital Heart Disease
Current Cardiology Reviews Self-assembly of Functional Molecular Shuttles Based on Organic Conjugated Molecules
Current Organic Chemistry Targets and Tools: Recent Advances in the Development of Anti-HCV Nucleic Acids
Infectious Disorders - Drug Targets