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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Inactivation Kinetics of β-N-Acetyl-D-glucosaminidase from Green Crab (Scylla serrata) by Guanidinium Chloride

Author(s): Ji-Ping Zhang, Bo Leng, Qian-Sheng Huang, Ya-Wen Yan, Xuan Liu, Qin Wang and Qing-Xi Chen

Volume 19, Issue 11, 2012

Page: [1177 - 1182] Pages: 6

DOI: 10.2174/092986612803217024

Price: $65

Abstract

β-N-acetyl-D-glucosaminidase (NAGase) is a major member in chitinolytic enzymes system, which plays an important role in the hatching and molting processes of marine organism. The effects of guanidinium chloride (GuHCl) on the activity of NAGase from green crab (Scylla serrata) were investigated in this study. In results, GuHCl causes reversible inactivation of the enzyme at below 0.8 M concentrations, and the IC50 is estimated to be 0.15 M. The relationship between the enzyme activity and conformation was charaterized by monitoring the change of protein fluorescence spectra. With increasing GuHCl concentration, the fluorescence intensity of the enzyme distinctly decreases , and the maximal emission peaks appear red-shifted (from 338 nm to 343 nm). The enzyme inactivation precedes conformational changes, indicating that the enzyme active site is more flexible than the whole enzyme molecule. The result of the kinetics of inactivation shows that the value of k+0 is larger than that of k+0 ' . It suggests that the substrate could protect the enzyme to a certain extent during guanidine denaturation. Our results provide important new insights in marine organism culture, especially in crustacean growth.

Keywords: Scylla serrata, β-N-acetyl-D-glucosaminidase, denaturation, inactivation kinetics, guanidinium chloride


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