Abstract
The peptide sequence KLVFFAE that spans the region 16-22 in the amyloid peptide Aβ1-40 has the ability to form fibrils or nanotubes in aqueous medium, depending on the conditions of dissolution. Interaction between the phenylalanine residues is presumed to play an important role in the self-assembly of Aβ16-22. We have investigated the importance of these aromatic residues by substituting them with p-chloro-, p-fluoro- and p-methylphenylalanine. Nanostructures different from the parent peptide were obtained with the substituted analogs, both in methanol as well as aqueous conditions (pH 2 and pH 7). Concentration-dependent effects observed in methanol, suggest that intermediate states occur during fibrillation. A balance between the crucial parameters such as charge, hydrophobicity and steric constraints implicated in self assembly, appear to modulate the nanostructure formation.
Keywords: Aβ16-22, aggregation, amyloid-like structure, derivatized-phenylalanines, nanostructures, self-assembly
Protein & Peptide Letters
Title:Effect of Replacing Phenylalanine Residues by Para-Substituted Phenylalanines on the Aggregation Behavior of Aβ16-22
Volume: 19 Issue: 5
Author(s): Chandrasekaran Sivakamasundari, Sasi Nandakumar and Ramakrishnan Nagaraj
Affiliation:
Keywords: Aβ16-22, aggregation, amyloid-like structure, derivatized-phenylalanines, nanostructures, self-assembly
Abstract: The peptide sequence KLVFFAE that spans the region 16-22 in the amyloid peptide Aβ1-40 has the ability to form fibrils or nanotubes in aqueous medium, depending on the conditions of dissolution. Interaction between the phenylalanine residues is presumed to play an important role in the self-assembly of Aβ16-22. We have investigated the importance of these aromatic residues by substituting them with p-chloro-, p-fluoro- and p-methylphenylalanine. Nanostructures different from the parent peptide were obtained with the substituted analogs, both in methanol as well as aqueous conditions (pH 2 and pH 7). Concentration-dependent effects observed in methanol, suggest that intermediate states occur during fibrillation. A balance between the crucial parameters such as charge, hydrophobicity and steric constraints implicated in self assembly, appear to modulate the nanostructure formation.
Export Options
About this article
Cite this article as:
Sivakamasundari Chandrasekaran, Nandakumar Sasi and Nagaraj Ramakrishnan, Effect of Replacing Phenylalanine Residues by Para-Substituted Phenylalanines on the Aggregation Behavior of Aβ16-22, Protein & Peptide Letters 2012; 19 (5) . https://dx.doi.org/10.2174/092986612800191044
DOI https://dx.doi.org/10.2174/092986612800191044 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Is Intraoperative Opioids Avoidance A Utopia? A Matched Study in Laparoscopic Hysterectomy
Current Reviews in Clinical and Experimental Pharmacology Synthetic and Medicinal Prospective of Structurally Modified Curcumins
Current Topics in Medicinal Chemistry Gender Hormones: Role in the Pathogenesis of Central Nervous System Disease and Demyelination
Current Neurovascular Research Treating Depression in Coronary Artery Disease and Chronic Heart Failure: What’s new in using Selective Serotonin Re-Uptake Inhibitors?
Cardiovascular & Hematological Agents in Medicinal Chemistry Blood-brain Barrier Disruption Leads to Postoperative Cognitive Dysfunction
Current Neurovascular Research Methylone and Monoamine Transporters: Correlation with Toxicity
Current Neuropharmacology Are B Cells a Potential Target for Therapeutic Intervention in the Classical T Cell-Mediated Autoimmune Disease Type 1 Diabetes?
Inflammation & Allergy - Drug Targets (Discontinued) Caveolin Involvement and Modulation in Breast Cancer
Mini-Reviews in Medicinal Chemistry Opioid Free Anaesthesia and Cancer
Current Pharmaceutical Design Pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidines and Structurally Simplified Analogs. Chemistry and SAR Profile as Adenosine Receptor Antagonists
Current Topics in Medicinal Chemistry Therapeutic Use of Vitamin D and its Analogues in Autoimmunity
Recent Patents on Inflammation & Allergy Drug Discovery DNA Secondary Structure at Chromosomal Fragile Sites in Human Disease
Current Genomics The Role of FKBP5 in Mood Disorders: Action of FKBP5 on Steroid Hormone Receptors Leads to Questions About its Evolutionary Importance
CNS & Neurological Disorders - Drug Targets Autophagy after Subarachnoid Hemorrhage: Can Cell Death be Good?
Current Neuropharmacology Preconditioning with Chronic Cerebral Hypoperfusion Reduces a Focal Cerebral Ischemic Injury and Increases Apurinic/Apyrimidinic Endonuclease/Redox Factor-1 and Matrix Metalloproteinase-2 Expression
Current Neurovascular Research Serotonergic Modulation of Spinal Sensory Circuits
Current Topics in Medicinal Chemistry Does Mechanism of Drug Action Matter to Inform Rational Polytherapy in Epilepsy?
CNS & Neurological Disorders - Drug Targets Gender, Hormones and the Transmissible Encephalopathies
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents Lysosomal Potassium Channels: Potential Roles in Lysosomal Function and Neurodegenerative Diseases
CNS & Neurological Disorders - Drug Targets The Role of Placental Carbonyl Reducing Enzymes in Biotransformation of Bupropion and 4-methylnitrosamino-1-(3-pyridyl)-1-butanone
Drug Metabolism Letters