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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Identification of Heterotrimeric G Protein α and β Subunits in Rice

Author(s): Yuki Abe, Keiko Matsusita, Setsuko Komatsu and Yukimoto Iwasaki

Volume 19, Issue 3, 2012

Page: [277 - 281] Pages: 5

DOI: 10.2174/092986612799363190

Price: $65


Like those in mammals, heterotrimeric G protein complexes have been implicated in signal transduction pathways in plants; however, the subunits themselves have not been isolated. In this study, the rice heterotrimeric G protein subunits α (Gα) and β (Gβ) were purified by affinity chromatography using anti-Gα and -Gβ antibodies and SDS-PAGE. Six and seven peptides, respectively, were identified by mass spectrometry and identified as the translation products of the Gα gene RGA1 and Gβ gene RGB1. During purification, the subunits dissociated easily from the G protein complex.

Keywords: Antibody, Gα subunit, Gβ subunit, heterotrimeric G protein, purification, recombinant protein, affinity chromatography, polyvinylidene difluoride, Arabidopsis, centrifugation

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