Molecular Probes for Bioluminescence Imaging

Song      Wu; Edwin      Chang; Zhen      Cheng

Abstract

Bioluminescence refers to the emission of light from a living system in which photoproteins such as luciferase enzymes oxidize their substrates to produce light. Because of its high-sensitivity and low-toxicity, bioluminescence imaging (BLI) is particularly useful for in vitro assays and in vivo small animal imaging. It provides a powerful tool to study various important biological questions and processes including gene and protein expression, protein-protein interactions, protein-nucleic acid interactions, and cell signaling pathway functions. This review highlights some of the latest developments in the design and applications of molecular probes for BLI.

Keywords: Bioluminescence imaging, Molecular Imaging, Probe, Luciferase, Bioluminescence Resonance Energy Transfer, MRI, Luciferase Mutugenesis, D-luciferin Analogs, Luciferase Fused Proteins, Intramolecular Enzyme Complementation