Abstract
Protein phosphorylation constitutes a major type of post-translational modifications mobilizing a high number of genes. It is involved in many crucial cell processes and largely participates in the features of the proteome. For several biological and technical reasons, the characterization of protein phosphorylation requires a combination of distinct procedures. In this review, a special emphasis is given to analytical strategies connected with the determination of the presence of phosphorylation sites and their localization by mass spectrometry (MS). The feasibility of selected combinations of analytical approaches for diverse objectives of phosphoproteomic research is discussed.
Keywords: Plant phosphorylation, phosphoproteomics, mass spectrometry, sample preparation, post-translational modifications, monitored protein separation, kinase inhibitors, spectrometric analysis., Liquid chromatography, lyophilization, Gel Electrophoresis, Chemical Tagging, Phospho-specific visualization, immunopurification
Current Protein & Peptide Science
Title: Mass Spectrometry Characterization of Plant Phosphoproteins
Volume: 12 Issue: 2
Author(s): Katerina Novakova, Ondrej Sedo and Zbynek Zdrahal
Affiliation:
Keywords: Plant phosphorylation, phosphoproteomics, mass spectrometry, sample preparation, post-translational modifications, monitored protein separation, kinase inhibitors, spectrometric analysis., Liquid chromatography, lyophilization, Gel Electrophoresis, Chemical Tagging, Phospho-specific visualization, immunopurification
Abstract: Protein phosphorylation constitutes a major type of post-translational modifications mobilizing a high number of genes. It is involved in many crucial cell processes and largely participates in the features of the proteome. For several biological and technical reasons, the characterization of protein phosphorylation requires a combination of distinct procedures. In this review, a special emphasis is given to analytical strategies connected with the determination of the presence of phosphorylation sites and their localization by mass spectrometry (MS). The feasibility of selected combinations of analytical approaches for diverse objectives of phosphoproteomic research is discussed.
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Cite this article as:
Novakova Katerina, Sedo Ondrej and Zdrahal Zbynek, Mass Spectrometry Characterization of Plant Phosphoproteins, Current Protein & Peptide Science 2011; 12 (2) . https://dx.doi.org/10.2174/138920311795684913
DOI https://dx.doi.org/10.2174/138920311795684913 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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