Abstract
Lung cancer is a leading cause of death in human. Cancer stem cells have been regarded as basis for failure of current therapeutic options. Salinomycin was shown to kill these cancer stem cells in some types of cancer such as breast cancer and leukemia. The in vitro anticancer activities of salinomycin have been validated against the lung cancer cell line A549 via sulforhodamine B and colony formation assay. Salinomycin has been demonstrated to significantly rupture the in vitro lung cancer tumorospheres from ALDH positive A549 lung cells using flow cytometry. Expression of stem cell markers OCT-4, NANOG and SOX2 in ALDH positive A549 lung cells was decreased significantly by real-time RT-PCR analysis after 24 hour salinomycin treatment. Taken together, salinomycin may provide a promising approach for lung cancer chemotherapy.
Keywords: A549 cells, cancer stem cell, lung adenocarcinoma, Salinomycin, anticancer activities, sulforhodamine B, colony formation assay, cancer tumorospheres, ALDH, flow cytometry, RT-PCR, 4-Oct, NANOG, SOX2, paclitaxel
Medicinal Chemistry
Title: Effects of Salinomycin on Cancer Stem Cell in Human Lung Adenocarcinoma A549 Cells
Volume: 7 Issue: 2
Author(s): Yi Wang
Affiliation:
Keywords: A549 cells, cancer stem cell, lung adenocarcinoma, Salinomycin, anticancer activities, sulforhodamine B, colony formation assay, cancer tumorospheres, ALDH, flow cytometry, RT-PCR, 4-Oct, NANOG, SOX2, paclitaxel
Abstract: Lung cancer is a leading cause of death in human. Cancer stem cells have been regarded as basis for failure of current therapeutic options. Salinomycin was shown to kill these cancer stem cells in some types of cancer such as breast cancer and leukemia. The in vitro anticancer activities of salinomycin have been validated against the lung cancer cell line A549 via sulforhodamine B and colony formation assay. Salinomycin has been demonstrated to significantly rupture the in vitro lung cancer tumorospheres from ALDH positive A549 lung cells using flow cytometry. Expression of stem cell markers OCT-4, NANOG and SOX2 in ALDH positive A549 lung cells was decreased significantly by real-time RT-PCR analysis after 24 hour salinomycin treatment. Taken together, salinomycin may provide a promising approach for lung cancer chemotherapy.
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Cite this article as:
Wang Yi, Effects of Salinomycin on Cancer Stem Cell in Human Lung Adenocarcinoma A549 Cells, Medicinal Chemistry 2011; 7 (2) . https://dx.doi.org/10.2174/157340611794859307
DOI https://dx.doi.org/10.2174/157340611794859307 |
Print ISSN 1573-4064 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6638 |
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