Furin Cleavage of Bacterial Expressed Glutathione-S-Transferase-Pro-Transforming Growth Factor β1 Fusion Protein In Vitro

Title: Furin Cleavage of Bacterial Expressed Glutathione-S-Transferase-Pro-Transforming Growth Factor β1 Fusion Protein In Vitro

Volume: 17 Issue: 4

Author(s): N. A. Kahle, C. Joffroy, S. L. Popp, C. Knabbe and M. B. Stope

Affiliation:

Keywords: Transforming growth factor β1, furin, proteolytic processing

Abstract: To investigate the processing of transforming growth factor β1 (TGFβ1) pro-protein by furin protease we expressed a GST-pro-TGFβ1 fusion protein in bacteria. Analysis of the furin digestion pattern revealed the liberation of 12.5 kDa TGFβ1 monomers. There was no evidence for cleavage of an alternative furin site within the pro-protein.

Cite this article as:

Kahle A. N., Joffroy C., Popp L. S., Knabbe C. and Stope B. M., Furin Cleavage of Bacterial Expressed Glutathione-S-Transferase-Pro-Transforming Growth Factor β1 Fusion Protein In Vitro, Protein & Peptide Letters 2010; 17 (4) . https://dx.doi.org/10.2174/092986610790963609