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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Resuscitation Promoting Factor Interacting Protein RipA from M. tuberculosis

Author(s): Alessia Ruggiero, Flavia Squeglia, Carla Esposito, Daniela Marasco, Emilia Pedone, Carlo Pedone and Rita Berisio

Volume 17, Issue 1, 2010

Page: [70 - 73] Pages: 4

DOI: 10.2174/092986610789909557

Price: $65

Abstract

RipA is an important growth factor of M. tuberculosis. Its depletion produces decreasing bacterial growth and abnormal phenotype. RipA C-terminal fragment (263-472), containing its predicted catalytic domain has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and atomic resolution refinement is in progress.

Keywords: Resuscitation promoting factor, crystal, growth factor, x-ray, tuberculosis


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