Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution

U.T.      Bornscheuer; D.      Bottcher; M.      Schmidt

Abstract

In the past few years a considerable number of mutagenesis methods and high-throughput screening (HTS) systems have been developed and improved. In parallel, computer programs or software packages for molecular modeling have been further investigated. Thus, the number of examples for successful directed evolution and rational design is increasing constantly. In this review the essential mutagenesis methods and HTS systems, especially for esterases, are described and various examples for the application of these protein engineering tools are provided.

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Protein & Peptide Letters

Title: Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution

Volume: 16 Issue: 10

Author(s): U.T. Bornscheuer, D. Bottcher and M. Schmidt

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Abstract: In the past few years a considerable number of mutagenesis methods and high-throughput screening (HTS) systems have been developed and improved. In parallel, computer programs or software packages for molecular modeling have been further investigated. Thus, the number of examples for successful directed evolution and rational design is increasing constantly. In this review the essential mutagenesis methods and HTS systems, especially for esterases, are described and various examples for the application of these protein engineering tools are provided.

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Bornscheuer U.T., Bottcher D. and Schmidt M., Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution, Protein & Peptide Letters 2009; 16 (10) . https://dx.doi.org/10.2174/092986609789071216