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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Characterisation of Oxidized Recombinant Human Galectin-1

Author(s): Stacy A. Scott, Andrea Bugarcic and Helen Blanchard

Volume 16, Issue 10, 2009

Page: [1249 - 1255] Pages: 7

DOI: 10.2174/092986609789071342

Price: $65


Oxidized human galectin-1 plays a role in the immune response to injured axons. Over-expression of galectin-1 by cancer, in combination with cancer associated oxidative stress suggests oxidized human galectin-1 may also play a role(s) in tumourigenesis. Here we generate milligram quantities of oxidized human galectin-1 and undertake biophysical characterization. The protein adopts a number of different states. Two separable oxidized forms are identified that exist as largely mono-disperse solutions at higher milligram/ml concentrations. The presence of disulphide bonds is confirmed for these two protein forms, as is their change in overall shape and loss of lectin activity. Our studies lead to production of a particular mono-disperse oxidized human galectin-1 species that is anticipated most optimal for investigations requiring milligram/ml concentrations such as X-ray crystallography.

Keywords: lectin activity, galectin-1 oxidation, protein redox state, Galectin-1

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