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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Immobilized Earthworm Fibrinolytic Enzyme III-1 with Carbonyldiimidazole Activated-agarose

Author(s): Xue-Qin Wu, Cen Wu and Rong-qiao He

Volume 9, Issue 1, 2002

Page: [75 - 80] Pages: 6

DOI: 10.2174/0929866023409039

Price: $65

Abstract

Earthworm fibrinolytic enzyme III-1 (EFE-III-1) was prepared to couple with cross-linking agarose activated by 1,1-Carbonyl-diimidazole (CDI) in this study. Although the activity of the immobilized protease decreased to approximately 64% of the native enzyme, the activity of EFE-III-1 coupled with the resin activated by CDI was higher than that activated by cyanogen bromide (CNBr). The immobilized protease was experimentally demonstrated to hydrolyze IgG, albumin and creatine kinase, besides fibrin(ogen) and plasmin(ogen), suggesting that EFE had a broad substrate specificity.

Keywords: Earthworm fibrinolytic enzyme III-1 (EFE-III-1), carbonyl-diimidazole (CDI), CDI-activated-Sepharose, Sepharose CL-6B, serine-protease


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