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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Author(s): Beatriz Dos Santos Ferreira, Alan Trindade Branco, Karlla Salim de Queiroz and Goncalo Apolinario de Souza Filho

Volume 9, Issue 1, 2002

Page: [39 - 43] Pages: 5

DOI: 10.2174/0929866023409057

Price: $65

Abstract

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

Keywords: isoelectric protein focusing (IEF), sodium dodecylsulfate (SDS)-PAGE, mercaptoethanol, Ampholytes solution, maize endosperm


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