Abstract
The phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) catalyzes the phosphorylation and transportation of its sugar substrates. A sugar-specific enzyme II complex involved in the PTS finally functions to translocate substrates across the membrane. A PTS EIIBfruc protein, a fructose specific EIIB subunit, from Escherichia coli has been cloned, expressed, refolded, purified, and crystallized. The synchrotron data were collected to 2.6 Å from the crystal of a selenomethionine substitute PTS EIIBfruc protein. The crystal belongs to the primitive trigonal space group P3121, with unit-cell parameters of a = 33.4 Å, b = 33.4 Å, c = 154.0 Å, and β = 120.0°. A full structure determination is under way to provide insights into the structure-function relationships of this protein.
Keywords: Phosphoenolpyruvate-carbohydrate phosphotransferase system, a fructose specific EIIB subunit, refolding, crystal, X-ray
Protein & Peptide Letters
Title: A Preliminary X-Ray Study of a Refolded PTS EIIBfruc Protein from Escherichia coli
Volume: 15 Issue: 6
Author(s): Dong Hae Shin
Affiliation:
Keywords: Phosphoenolpyruvate-carbohydrate phosphotransferase system, a fructose specific EIIB subunit, refolding, crystal, X-ray
Abstract: The phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) catalyzes the phosphorylation and transportation of its sugar substrates. A sugar-specific enzyme II complex involved in the PTS finally functions to translocate substrates across the membrane. A PTS EIIBfruc protein, a fructose specific EIIB subunit, from Escherichia coli has been cloned, expressed, refolded, purified, and crystallized. The synchrotron data were collected to 2.6 Å from the crystal of a selenomethionine substitute PTS EIIBfruc protein. The crystal belongs to the primitive trigonal space group P3121, with unit-cell parameters of a = 33.4 Å, b = 33.4 Å, c = 154.0 Å, and β = 120.0°. A full structure determination is under way to provide insights into the structure-function relationships of this protein.
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Cite this article as:
Shin Hae Dong, A Preliminary X-Ray Study of a Refolded PTS EIIBfruc Protein from Escherichia coli, Protein & Peptide Letters 2008; 15 (6) . https://dx.doi.org/10.2174/092986608784967001
DOI https://dx.doi.org/10.2174/092986608784967001 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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