Production, Purification and Characterization of β-1,4-Endoglucanase from a Novel Bacterial Strain CTP-09 of a Bacillus sp.

Mahjabeen      Saleem; Muhammad   Saleem   Akhtar; Riffat      Yasmin; Mahreen      Zahid; Nadeem   Nawazish   Malik; Munazza      Afzal; Muhammad   Ibrahim   Rajoka

Abstract

Bacillus strain CTP-09 yielded maximum productivity (1120 IU/L.h) of extracellular endoglucanase (CMCase) on 0.5% cellobiose after 10 h fermentation at 55° C. The purified enzyme is mono-meric in nature and exhibits stability up to 80° C and over a pH range (6.0-9.0). Activation energy, enthalpy and entropy of catalysis, and inactivation indicated that this CMCase is highly thermos-table. Purified enzyme possessed high power of defibrillation of textile and was minutely inhibited by anionic detergent and oxidizing agent comparable with inhibition by commercial enzyme. This polypeptide could be exploited for mass production and application in local industries.

Keywords: Bacillus sp., endoglucanase, enthalpy, entropy, kinetics, thermodynamics, turn over number

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Protein & Peptide Letters

Title: Production, Purification and Characterization of β-1,4-Endoglucanase from a Novel Bacterial Strain CTP-09 of a Bacillus sp.

Volume: 15 Issue: 4

Author(s): Mahjabeen Saleem, Muhammad Saleem Akhtar, Riffat Yasmin, Mahreen Zahid, Nadeem Nawazish Malik, Munazza Afzal and Muhammad Ibrahim Rajoka

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Keywords: Bacillus sp., endoglucanase, enthalpy, entropy, kinetics, thermodynamics, turn over number

Abstract: Bacillus strain CTP-09 yielded maximum productivity (1120 IU/L.h) of extracellular endoglucanase (CMCase) on 0.5% cellobiose after 10 h fermentation at 55° C. The purified enzyme is mono-meric in nature and exhibits stability up to 80° C and over a pH range (6.0-9.0). Activation energy, enthalpy and entropy of catalysis, and inactivation indicated that this CMCase is highly thermos-table. Purified enzyme possessed high power of defibrillation of textile and was minutely inhibited by anionic detergent and oxidizing agent comparable with inhibition by commercial enzyme. This polypeptide could be exploited for mass production and application in local industries.

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Saleem Mahjabeen, Akhtar Saleem Muhammad, Yasmin Riffat, Zahid Mahreen, Malik Nawazish Nadeem, Afzal Munazza and Rajoka Ibrahim Muhammad, Production, Purification and Characterization of β-1,4-Endoglucanase from a Novel Bacterial Strain CTP-09 of a Bacillus sp., Protein & Peptide Letters 2008; 15 (4) . https://dx.doi.org/10.2174/092986608784246560