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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

DNA Cleavage Study Using Copper (II)-GlyAibHis: A Tripeptide Complex Based on ATCUN Peptide Motifs

Author(s): Udai Pratap Singh, Raj Kumar Singh, Nilesh Kumar Sharma and Ramasre Prasad

Volume 15, Issue 1, 2008

Page: [13 - 19] Pages: 7

DOI: 10.2174/092986608783330378

Price: $65

Abstract

Development of new chemical nucleases is a matter of great interest because of their extensive use in biotechnology and as therapeutic agents. The ATCUN (amino terminal Cu(II) and Ni(II) binding) is a peptide motif that occurs naturally in the serum albumins. The similar peptide motif (GlyAibHis) having unnatural amino acid Aib (α- aminoisobutyric acid) was synthesized and its Cu(II) complex was characterized by ESI-MS and spectrophotometry studies. The reactivity of this complex toward DNA cleavage has been investigated. Cu(II)-GlyAibHis shows the DNA cleavage only in presence of mild oxidizing agents like ascorbate by an oxidative mechanism rather than hydrolytic and follows the pseudo first order kinetics (Kobs = 0.085 min-1). The non-hydrolytic mechanism was further supported by the hydrolysis of pNPP which followed the pseudo first order kinetics (Kobs = 1.98 x 10-2 min-1) having no pH effect.

Keywords: Solid phase synthesis, Calf thymus DNA, Circular Dichroism, Ester Hydrolysis, para-nitrophenylphosphate


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