Abstract
To elucidate potential biological roles of human β-synuclein, we studied its conformational changes under various conditions. The structural and functional properties of β-synuclein were characterized using biochemical and biophysical methods including: a functional assay, mass spectrometry, size exclusion chromatography, circular dichroism (CD), and fluorescence spectroscopy. The results showed β-synuclein has a high proportion of random coil in solution.
Keywords: β-Synuclein, circular dichroism, fluorescence, conformational change
Protein & Peptide Letters
Title: Spectroscopic and Functional Characterization of Human β-Synuclein
Volume: 14 Issue: 10
Author(s): Juhyung Lee, Heejin Hwang and T. Doohun Kim
Affiliation:
Keywords: β-Synuclein, circular dichroism, fluorescence, conformational change
Abstract: To elucidate potential biological roles of human β-synuclein, we studied its conformational changes under various conditions. The structural and functional properties of β-synuclein were characterized using biochemical and biophysical methods including: a functional assay, mass spectrometry, size exclusion chromatography, circular dichroism (CD), and fluorescence spectroscopy. The results showed β-synuclein has a high proportion of random coil in solution.
Export Options
About this article
Cite this article as:
Lee Juhyung, Hwang Heejin and Kim Doohun T., Spectroscopic and Functional Characterization of Human β-Synuclein, Protein & Peptide Letters 2007; 14 (10) . https://dx.doi.org/10.2174/092986607782541132
DOI https://dx.doi.org/10.2174/092986607782541132 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Disentangling the Role of the Tau Gene Locus in Sporadic Tauopathies
Current Alzheimer Research Update on the Important New Drug Target in Cardiovascular Medicine – the Vascular Glycocalyx
Cardiovascular & Hematological Disorders-Drug Targets Aptamer-Based Targeted Drug Delivery Systems: Current Potential and Challenges
Current Medicinal Chemistry Effects of Acute and Chronic Treatment of Novel Psychotropic Drug, 8- (Trifluoromethyl)-1, 2, 3, 4, 5-benzopentathiepin-6-amine Hydrochloride (TC-2153), on the Behavior of Zebrafish (Danio Rerio): A Comparison with Fluoxetine
Letters in Drug Design & Discovery Memantine and Kynurenic Acid: Current Neuropharmacological Aspects
Current Neuropharmacology Sulfur Containing Scaffolds in Drugs: Synthesis and Application in Medicinal Chemistry
Current Topics in Medicinal Chemistry Effects of MDMA on Extracellular Dopamine and Serotonin Levels in Mice Lacking Dopamine and/or Serotonin Transporters
Current Neuropharmacology Advanced Glycation End Products, Oxidative Stress and Metalloproteinases are altered in the Cerebral Microvasculature during Aging
Current Neurovascular Research Use of Peripheral Blood Stem Cells in Tissue Engineering
Current Tissue Engineering (Discontinued) An Update on the Role of Nitric Oxide in the Neurodegenerative Processes of Parkinson's Disease
Current Medicinal Chemistry Xanthine Derivatives as Agents Affecting Non-dopaminergic Neuroprotection in Parkinson’s Disease
Current Medicinal Chemistry Short, but Highly Concentrated, Direct Inhalations of Essential Oil Molecules Improve Poor Sleep Quality: A Natural History Controlled, Withingroup, Self-administration Field Pilot Study
Current Psychopharmacology Cognitive Improvement by Activation of α7 Nicotinic Acetylcholine Receptors: From Animal Models to Human Pathophysiology
Current Pharmaceutical Design Editorial (Thematic Issue: Rational Approaches for the Design of Monoamine Oxidase Inhibitors)
Central Nervous System Agents in Medicinal Chemistry Homo obesus: A Metabotrophin-Deficient Species. Pharmacology and Nutrition Insight
Current Pharmaceutical Design The Expanding Universe of Neurotrophic Factors: Therapeutic Potential in Aging and Age-Associated Disorders
Current Pharmaceutical Design Generalized String Pseudo-Folding Lattices in Bioinformatics: State-of-Art Review, New Model for Enzyme Sub-Classes, and Study of ESTs on Trichinella spiralis
Current Bioinformatics Spectral Imaging Technology - A Review on Skin and Endoscopy Applications
Recent Patents on Medical Imaging Functional Roles of Synaptic and Extrasynaptic NMDA Receptors in Physiological and Pathological Neuronal Activities
Current Drug Targets Targeted Radionuclide Therapy of Painful Bone Metastases: Past Developments, Current Status, Recent Advances and Future Directions
Current Medicinal Chemistry