Cloning and Expression of a Novel Long Neurotoxin from Bungarus multicinctus in E. coli

Qin      Cai; Sheng-li      Yang; Yi      Gong

Abstract

The total RNA was isolated from Bungarus multicinctus venom. A novel long neurotoxin (LNT) cDNA was amplified by RT-PCR method. Sequence analysis revealed that the cDNA of long neurotoxin has an open reading frame encoding 94 amino acids, including a signal peptide of 21 amino acids. The mature peptide encoding sequence was inserted into plasmid pET-28 (a+), and then which was expressed in the E. coli BL21 (DE3). The expressed Protein is identified as a novel long neurotoxin by western blot.

Keywords: NEUROTOXIN, total RNA, neurotoxin (LNT) cDNA

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Protein & Peptide Letters

Title: Cloning and Expression of a Novel Long Neurotoxin from Bungarus multicinctus in E. coli

Volume: 8 Issue: 3

Author(s): Qin Cai, Sheng-li Yang and Yi Gong

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Keywords: NEUROTOXIN, total RNA, neurotoxin (LNT) cDNA

Abstract: The total RNA was isolated from Bungarus multicinctus venom. A novel long neurotoxin (LNT) cDNA was amplified by RT-PCR method. Sequence analysis revealed that the cDNA of long neurotoxin has an open reading frame encoding 94 amino acids, including a signal peptide of 21 amino acids. The mature peptide encoding sequence was inserted into plasmid pET-28 (a+), and then which was expressed in the E. coli BL21 (DE3). The expressed Protein is identified as a novel long neurotoxin by western blot.

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Cai Qin, Yang Sheng-li and Gong Yi, Cloning and Expression of a Novel Long Neurotoxin from Bungarus multicinctus in E. coli, Protein & Peptide Letters 2001; 8 (3) . https://dx.doi.org/10.2174/0929866013409508