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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Kinetic and Site-Directed Mutagenesis Studies of Prevotella Intermedia Acid Phosphatase

Author(s): Xiaochi Chen, Toshihiro Ansai, Sailen Barik and Tadamichi Takehara

Volume 10, Issue 1, 2003

Page: [53 - 59] Pages: 7

DOI: 10.2174/0929866033408282

Price: $65


Site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from Prevotella intermedia (PiACP). The replacement of histidine and arginine residues resulted in the elimination of the PiACP activity while the cysteine mutants retained activity. These results suggest that the histidine and arginine residues are essential for catalysis.

Keywords: prevotella intermedia acp, piacp, acid phosphatase, site-directed mutagenesis

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