Kinetic and Site-Directed Mutagenesis Studies of Prevotella Intermedia Acid Phosphatase

Title: Kinetic and Site-Directed Mutagenesis Studies of Prevotella Intermedia Acid Phosphatase

Volume: 10 Issue: 1

Author(s): Xiaochi Chen, Toshihiro Ansai, Sailen Barik and Tadamichi Takehara

Affiliation:

• Department of Preventive Dentistry, School of Stomatology, Peking University,22 Zhongguancun Nandajie Haidian District, Beijing 1000811 China,China

Keywords: prevotella intermedia acp, piacp, acid phosphatase, site-directed mutagenesis

Abstract: Site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from Prevotella intermedia (PiACP). The replacement of histidine and arginine residues resulted in the elimination of the PiACP activity while the cysteine mutants retained activity. These results suggest that the histidine and arginine residues are essential for catalysis.

Cite this article as:

Chen Xiaochi, Ansai Toshihiro, Barik Sailen and Takehara Tadamichi, Kinetic and Site-Directed Mutagenesis Studies of Prevotella Intermedia Acid Phosphatase, Protein & Peptide Letters 2003; 10(1) . https://dx.doi.org/10.2174/0929866033408282