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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Inhibition of Human Napsin A

Author(s): Rebecca F. Cronshaw, Vesna Schauer-Vukasinovic, David J. Powell, Thomas Giller, Daniel Bur and John Kay

Volume 10, Issue 1, 2003

Page: [35 - 42] Pages: 8

DOI: 10.2174/0929866033408237

Price: $65


The newly-discovered human aspartic proteinase, napsin A was not susceptible to protein inhibitors from potato, squash or yeast but was weakly inhibited by the 17 kDa polypeptide from Ascaris lumbricoides and potently by isovaleryl and lactoyl-pepstatins. A series of synthetic inhibitors was also investigated which contained in the P1-P1 positions the dipeptide analogue statine or its phenylalanine or cyclohexylalanine homologues and in which the residues occupying P4-P3 were varied systematically. On this basis, the active site of napsin A can be readily distinguished from other human aspartic proteinases.

Keywords: human aspartic proteinase, selectivity of inhibition, active site mapping, importance of s1/s3, hapsin a, napsin a inhibitor

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